Product
제품
- AlbuVoid™
- 제품명: AlbuVoid™
- 용도: Albumin & IgG Removal Kits
- 메이커: Biotech Support Group
- 카달로그:
소개
- 알부민 제거율 >95% (30분 이내)
- 단백질 구조 및 활성 보존
- 다양한 분석 플랫폼과 호환
- 종 특이성 없음
- 분자량 및 pl 편향 없음
- BASP™ 기반 On-bead workflow 지원
- iTRAQ 및 label-free LC-MS 호환
AlbuVoid™는 혈청 및 혈장 시료에서 알부민을 제거하면서 저농도 및/또는 저분자량 단백질을 농축할 수 있도록 설계된 시약 키트입니다. 비변성적 버퍼(mild buffers) 조건을 사용하므로, 용출 분획에서 단백질의 구조 및 native 효소 활성이 유지됩니다. 또한 기존 농축 방법의 한계로 지적되는 50kDa 이하 단백질의 분해능을 크게 향상시킬 수 있습니다.
알부민과 결합하지 않도록 설계되어 있으며, 사료 내 다른 단백질들은 AlbuVoid™에 결합한 뒤 용출됩니다. 그 결과 알부민은 제거되고, 저농도 혈청 단백질이 선택적으로 농축됩니다. 이러한 특성은 바이오마커 발굴, 효소 분석, 신약 독성 연구, SELDI 기반 단백질 프로파일링, 단백질 어레이, 1D/2D 전기영동, LC-MS, MALDI-TOF MS 및 사이토카인 연구에 유용합니다.
* AlbuTrial™ 키트 제공: Albusorb™ 1g 및 AlbuVoid™ 5회분 구성
[제품 프로토콜 개요]
사양
주문정보
| Product | Size | Total samples processed | Item No. |
|---|---|---|---|
| AlbuVoid™ | 10 preps | 10 preps x 100 - 200 µl samples | AVK-10 |
| AlbuVoid™ | 50 preps | 50 preps x 100 - 200 µl samples | AVK-50 |
| AlbuTrial Kit™ |
AlbuVoid™, 5 Preps AlbuSorb™, 20 preps |
5 preps x 200 µl samples (AlbuVoid) 20 preps x 25 µl samples (AlbuSorb) |
AVS-05 |
| Items Required | 5 Prep | 10 Prep | 50 Prep | Reagent |
|---|---|---|---|---|
| AlbuVoid™ | 0.25 gram | 0.5 gram | 2.5 gram | Supplied |
| Binding Buffer AVBB, PH 6.0 | 4 ml | 7 ml | 35 ml | Supplied |
| Wash Buffer AVWB, PH 7.0 | 5 ml | 10 ml | 50 ml | Supplied |
| Elution Buffer AVEB, PH 9.8 | 2 ml | 4 ml | 20 ml | Supplied |
| SpinX Centrifuge tube filters | 5 | 10 | 50 | Supplied |
관련자료
Selection of AlbuVoid™ References
Paulsen, Bruna, et al. "Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment." bioRxiv (2024): 2024-04.
The investigators performed secretome proteomics to investigate proteins overexpressed in OSC. The conditioned media was passed through albumin depletion columns (AlbuVoid) to eliminate HSA-derived albumin.
Jenull, Sabrina, et al. "The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence." Cell Reports 36.3 (2021): Link.
Cell-free supernatants were used for Mass-Spec analysis. Collected supernatants were treated with AlbuVoid™ for albumin depletion; Albumin-free enriched secretory proteome was eluted from beads.
Paulsen et al., Development of human induced pluripotent stem cell-derived ovarian support cells as a clinical-grade product for in vitro fertilization, Cell Stem Cell (2025).
AlbuVoid™ enrichment helped in characterizing key functionalities of hiPSC-derived cell lines.
Golden, Lesley R., et al. "APOE4 to APOE2 allelic switching in mice improves Alzheimer’s disease-related metabolic signatures, neuropathology and cognition." Nature Neuroscience (2025).
ApoE enrichment with AlbuVoid™ helped in identifying the closely related ApoE4/2 variants by targeted plasma proteomic analysis.
Sae-Lee, Wisath, et al. "The protein organization of a red blood cell." Cell Reports 40.3 (2022).
The supernatant was treated with AlbuVoid to reduce the presence of serum albumin for plasma preparation.
Vialaret, Jerome, et al. "Albumin depletion of human serum to improve quantitative clinical proteomics." Current Topics in Peptide & Protein Research (2018).
AlbuVoid™ depletion method proved to be faster and more cost-effective than antibody based methods for biomarker enrichment.
Jing, Lun, et al. "PROTEOMIC ANALYSIS IDENTIFIED LBP AND CD14 AS KEY PROTEINS IN BLOOD/BIPHASIC CALCIUM PHOSPHATE MICROPARTICLE INTERACTIONS." Acta Biomaterialia (2021).
Describes use of HemoVoid™ and AlbuVoid™ prior to LC-MS analysis to identify changes in 27 signaling pathways.
Poillet-Perez, Laura, et al. "Autophagy maintains tumour growth through circulating arginine." Nature (2018).
AlbuVoid™ was used to remove albumin from mice serum and enrich low abundance proteomes to study autophagy.
Zheng H, et al. "AlbuVoid™ Coupled to On-Bead Digestion – Tackling the Challenges of Serum Proteomics." J Proteomics Bioinform (2015).
Methods gain workflow efficiencies over other high abundance depletion and in-solution digestion workflows.
Swapan Roy, Matthew Kuruc. "The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery." MOJ Proteomics Bioinform (2016).
Demonstrates utility of AlbuVoid™ & AlbuSorb™ to satisfy investigations of serum SERPINs.
David L. Wang, et al. "Identification of potential serum biomarkers for breast cancer using a functional proteomics technology." Biomarker Research (2017).
AlbuVoid™ was necessary to enrich low-level proteases to detectable levels for enzyme activity detection.
For a full list of Albumin Removal references, visit: BSG Albumin Depletion References