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- AlbuSorb™ PLUS
- 제품명: AlbuSorb™ PLUS
- 용도: Albumin & IgG Removal Kits
- 메이커: Biotech Support Group
- 카달로그:
소개
- 25 µl 혈청 샘플에서 알부민 및 IgG 85% 이상 제거
- 최적화된 Protein A와 고분자 전해질(Poly-electrolytes) 비드 기술의 결합
- 항체 친화성 방식과 동등한 선택성 및 낮은 비특이적 반응
- 온화한 조건으로 단백질 구조 유지 및 생물학적 활성 보존
- 엑소좀(EV) 화물 농축 및 프로테오믹스 분석에 최적화
- 일회용 시약으로 교차 오염 및 배치 간 편차 원천 차단
- LC-MS, 웨스턴 블롯, 1D/2D 전기영동 등 하위 분석과 완벽 호환
AlbuSorb™ PLUS시료 처리 후 원심분리 또는 여과를 통해 알부민과 면역글로불린이 제거된 혈청 분획을 여과액 형태로 회수합니다. 또한 Blue-dye affinity에 기반하지 않은 고유 비드 기술과 최적화된 Protein A를 결합한 솔루션으로, 면역분석, Western blot, 1D/2D 전기영동, 효소 분석, LC-MS 등 다양한 후속 분석에 폭 넓게 활용할 수 있습니다.
[AlbuSorb™ PLUS 프로토콜 개요]
사양
주문정보
관련자료
Cerebrospinal Fluid
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
Synovial fluid
Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex. Journal of Biological Chemistry.2012;287(11):8092-100
Serum
Zheng H, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™ Enrichment & Antibody Depletion – Tackling the Challenges of Serum Proteomics Part II. Poster reprint first presented at 26th International Molecular Med TRI-CON, March 10-15, 2019, San Francisco, CA USA
Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.
Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33
Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis
Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
Urine
Zubiri, Irene, et al. "Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis." Journal of proteomics 96 (2014): 92-102.
Patent
Berggren, Per Olaf, Yang, Shao-Nian. 2012. Methods For Treating And/Or Limiting Development Of Diabetes.U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, and issued December 27, 2012.