NRicher™ Apo

아포지단백질(apolipoproteins)의 선택적 농축

개인맞춤형 헬스케어를 뒷받침할 새로운 바이오마커에 대한 요구가 증가함에 따라, 최근 수년간 프로테오믹스 분야에서는 다양한 기술 혁신이 이루어져 왔습니다.그럼에도 불구하고 여전히 몇 가지 중요한 과제가 남아 있습니다. 그중 하나는 동적 범위(dynamic range) 문제로, 고농도 단백질이 저농도의 잠재적 바이오마커 단백질을 가려 검출을 어렵게 할 수 있습니다. 또 다른 과제는 표적 프로테오믹스 워크플로우(targeted proteomic workflows)입니다.서로 다른 샘플 코호트 간 측정의 효율성과 일관성이 충분히 확보되지 않는 문제가 있으며, 이는 샘플마다 단백질 및 펩타이드 조성이 달라지기 때문입니다. NRicher™ 제품군은 이러한 두 가지 문제를 모두 해결할 수 있도록 설계되었습니다.

15년 이상 BSG는 상이한 프로테옴 결합 특성을 갖는 합성 비드 개발 분야를 선도해 왔습니다.이러한 비드는 이온성, 소수성, 수소결합성, 방향족 및 고분자 기반 특성을 포함합니다. 최근에는 면역친화법을 대체하기 위해 자기 나노입자 기반 코로나 분리 기술이 도입되었으나, 복잡한 워크플로우와 다중 LC-MS 분석이 필요합니다. NRicher™는 이러한 한계를 극복하여 보다 단순하고 다목적인 솔루션을 제공합니다.

NRicher™ 플랫폼은 정밀한 서브프로테옴 농축을 통해 프로테오믹스 워크플로우의 효율성과 생산성을 향상시킵니다. 비드 화학 최적화와 버퍼 개선을 통해 분석 시간 단축과 비용 효율성 확보가 가능합니다.

Product	Size	Total serum/plasma samples processed	Item No.
NRicher™ APO	10 Preps	10 preps x (25-50) µl samples	NAPO-10
NRicher™ APO	50 Preps	50 preps x (25-50) µl samples	NAPO-50

Highlights from Biotech Support Group Exhibiting at World Human Proteome Congress

Biotech Support Group (BSG) exhibited at the 2025 World Human Proteome (HUPO) Congress, November 9-13, 2025 in Toronto Canada.NRicher™ Beads – for targeted serum proteomic biomarker enrichment, simplified and diversified workflows.The NRicher™ Knowledgebase, for bead enrichment of user-selected targets

 

New Jersey Commission On Science, Innovation And Technology Phase II Catalyst Research Grant Awarded to Biotech Support Group

Biotech Support Group announces receipt of a NEW JERSEY COMMISSION ON SCIENCE, INNOVATION AND TECHNOLOGY Phase II Catalyst Research Award of $40,000.The Research Award entitled “Evaluating the NRicher™ Bead Platform for Targeted LC-MS Quantification” will be conducted at The Rutgers Biological Mass Spectrometry Facility.This award follows from the Catalyst Phase I Award demonstrating simple workflows that generate low abundance enrichment of sub-proteomes suitable for quantitative plasma proteomics.

 

Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al. (2024)ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii.PLoS Comput Biol 20(6): e1012208.https://doi.org/10.1371/journal.pcbi.1012208

The article states we used affinity beads (NuGel PROspector) to pre-enrich Toxoplasma gondii lysate to capture five distinct subproteomes.[Note: NuGel PROspector beads are now part of the NRicher™ platform.]When comparing the 5 different subproteomes, there is clearly different selection biases amongst the 5 surface chemistries.Also, many of the proteins observed from the NRicher™ beads were not observed in the ion exchange fractions, demonstrating the importance of combining different modes (ionic, hydrophobic, etc.) of separation to improve overall proteome coverage.

 

Efficiencies gained in targeted serum proteomics using NRicher Beads – simplified and diversified workflows for sub-proteome and biomarker enrichment – Poster HUPO World Congress 2024

After NRicher™ sample prep, target peptides have highly enhanced spectral signal.NRicher™ sub-proteome enrichment can minimize acquisition time, improving throughput, cost, and productivity.Specific target peptides that report functional PTMs and amino acid variant regions promise insights and potential multiplex biomarkers for disease.

https://www.biotechsupportgroup.com/v/vspfiles/tem...

 

Wan C., Borgeson B., Phanse S. et al.Panorama of ancient metazoan macromolecular complexesNature 525, 339–344 (2015).

Six different NRicher™ beads (described with an old tradename PROspector) were used as an enrichment step in the workflow;about twice the number of observations and annotations became possible.This validates that the sub-proteome bias characteristics of the NRicher™ platform can simplify complex proteomes into enriched sub-proteomes for deep functional characterization.

 

Whitepaper - NRicher™: A Low Abundance Proteome Enrichment Platform With Seamless Integration of On-Bead Digestion

The NRicher™ Advantage:• Consumable chemically derived NuGel™ beads (species agnostic)• No specialized instruments required (standard microfuge only)• Bead cocktails enable single LC-MS analysis• Functionally active sub-proteomes for enzymatic and immunoassay analysis

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/BiotechSupportGroup-NRicher-Whitepaper.pdf

 

NRicher: Family Specific Enrichment For Targeted Proteomics – Poster USHUPO 2024

The need for new biomarkers to support personalized healthcare has fostered numerous proteomic innovations.Still, challenges remain, including high abundance proteins and inconsistency in quantifying target peptides across cohorts.NRicher provides a solution to both challenges.

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/NRicher%20poster%20small.pdf