NRicher™

저농도 프로테옴(Proteome) 농축 키트

개인맞춤형 헬스케어를 뒷받침할 새로운 바이오마커에 대한 요구가 증가함에 따라, 최근 수년간 프로테오믹스 분야에서는 다양한 기술 혁신이 이루어져 왔습니다. 그럼에도 불구하고 여전히 몇 가지 중요한 과제가 남아 있습니다. 그중 하나는 동적 범위(dynamic range) 문제로,고농도 단백질이 저농도의 잠재적 바이오마커 단백질을 가려 검출을 어렵게 할 수 있다. 또 다른 과제는 표적 프로테오믹스 워크플로우(targeted proteomic workflows)입니다. 즉, 관심 있는 바이오마커가 이미 알려져 있는 경우에도, 서로 다른 샘플 코호트 간 측정의 효율성과 일관성이 충분히 확보되지 않는 문제가 있습니다. 이는 선택된 바이오마커와 직접 관련이 없는 단백질 및 펩타이드 조성이 샘플마다 달라지기 때문이기도 합니다.NRicher™ 제품군은 이러한 두 가지 문제를 모두 해결할 수 있도록 설계되었습니다.

15년 이상 BSG 는 상이한 프로테옴 결합 특성을 갖는 합성 비드 개발 분야를 선도해 왔습니다. 이러한 비드는 이온성, 소수성, 수소결합성, 방향족 및 고분자 기반 특성을 포함하며, 이를 기반으로 한 다양한 파생 제품은 주요 학술지에 다수 인용되며 학계에서 널리 활용되고 있습니다. 최근에는 면역친화법을 대체하기 위한 접근으로, 자기 나노입자에 적용된 코로나 기반 분리 기술이 도입되었습니다. 그러나 이 방법은 특수 장비 기반의 복잡한 워크플로우와 더불어, 여러 비드 유래 서브프로테옴에 대한 개별 LC-MS 분석을 요구합니다. NRicher™ 는 이러한 한계를 극복하여 보다 단순하고 다목적으로 활용 가능한 솔루션을 제시합니다.

NRicher™ 플랫폼은 정밀한 서브프로테옴 농축을 통해 프로테오믹스 워크플로우의 효율성과 생산성을 향상시킬 수 있는 가능성을 제공합니다.응용 목적에 맞게 설계된 비드 화학을 활용할 수 있으며, 버퍼 최적화와 같은 공정 개선을 통해 워크플로우를 단순화하고 분석 시간을 단축하며 비용 효율성을 높일 수 있습니다. 이러한 개발은 NRicher™ Knowledgebase에 의해 지원됩니다.

Product	Size	Total serum/plasma samples processed	Item No.
NRicher™ Mx	10 Preps	10 preps × (2–4 mg total protein samples)	NIMX-10
NRicher™ Mx	50 Preps	50 preps × (2–4 mg total protein samples)	NIMX-50

Highlights from Biotech Support Group Exhibiting at World Human Proteome Congress

Biotech Support Group (BSG) exhibited at the 2025 World Human Proteome (HUPO) Congress, November 9-13, 2025 in Toronto Canada. NRicher™ Beads – for targeted serum proteomic biomarker enrichment, simplified and diversified workflows. The NRicher™ Knowledgebase, for bead enrichment of user-selected targets

New Jersey Commission On Science, Innovation And Technology Phase II Catalyst Research Grant Awarded to Biotech Support Group

Biotech Support Group announces receipt of a NEW JERSEY COMMISSION ON SCIENCE, INNOVATION AND TECHNOLOGY Phase II Catalyst Research Award of $40,000. The Research Award entitled “Evaluating the NRicher™ Bead Platform for Targeted LC-MS Quantification” will be conducted at The Rutgers Biological Mass Spectrometry Facility. This award follows from the Catalyst Phase I Award demonstrating simple workflows that generate low abundance enrichment of sub-proteomes suitable for quantitative plasma proteomics.

Swapna LS, Stevens GC, Sardinha-Silva A, Hu LZ, Brand V, Fusca DD, et al. (2024)ToxoNet: A high confidence map of protein-protein interactions in Toxoplasma gondii.PLoS Comput Biol 20(6): e1012208.https://doi.org/10.1371/journal.pcbi.1012208

The article states we used affinity beads (NuGel PROspector) to pre-enrich Toxoplasma gondii lysate to capture five distinct subproteomes. [Note: NuGel PROspector beads are now part of the NRicher™ platform.]. When comparing the 5 different subproteomes, there is clearly different selection biases amongst the 5 surface chemistries. Also, many of the proteins observed from the NRicher™ beads, were not observed in the Ion exchange fractions demonstrating the importance of combining different modes (ionic, hydrophobic, etc.) of separation to alter selection properties, and consequently improving overall proteome coverage.

Efficiencies gained in targeted serum proteomics using NRicher Beads – simplified and diversified workflows for sub-proteome and biomarker enrichment – Poster HUPO World Congress 2024

After NRicher™ sample prep, target peptides have highly enhanced spectral signal. NRicher™ sub-proteome enrichment can minimize acquisition time, collectively improving overall throughput, cost, and productivity. Specific target peptides that report functional PTMs and amino acid variant regions promise insights and potential multiplex biomarkers for disease.

https://www.biotechsupportgroup.com/v/vspfiles/tem...

Wan, C., Borgeson, B., Phanse, S. et al.Panorama of ancient metazoan macromolecular complexesNature 525, 339–344 (2015). https://doi.org/10.1038/nature14877

Six different NRicher™ beads (described with an old tradename PROspector) were used as an enrichment step in the overall workflow; about twice the number of observations and annotations became possible. This further validates that the sub-proteome bias characteristics of the NRicher™ surface chemistry platform can simplify complex proteomes into enriched sub-proteomes with efficiencies suitable for deep functional proteome characterization.

Whitepaper - NRicher™: A Low Abundance Proteome Enrichment Platform With Seamless Integration of On-Bead Digestion

The NRicher™ Advantage is described:
• Consumable chemically derived NuGel™ beads, species agnostic as they are not derived from antibodies
• Does not require any specialized instruments, just a standard microfuge
• Use of bead cocktails allows for one, rather than multiple LC-MS analyses
• Functionally active sub-proteomes after separations, for any orthogonal functional, enzymatic, or immunoassay analysis

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/BiotechSupportGroup-NRicher-Whitepaper.pdf

NRicher: Family Specific Enrichment For Targeted Proteomics – Poster USHUPO 2024

The need for new biomarkers to support personalized healthcare has fostered numerous proteomic innovations. Still, a number of challenges remain. One is the preponderance of high abundance proteins and, concurrently in targeted proteomic workflows, efficiency and consistency in quantifying target peptides from different sample cohorts. This is in part due to the changing landscape of proteins/peptides not associated with the selected targets. A solution for both these challenges is now available through a suite of products called NRicher.

https://www.biotechsupportgroup.com/v/vspfiles/templates/257/pdf/NRicher%20poster%20small.pdf