DynaPro™ Plate Reader 4     

 

High-Throughput, Automated, Dynamic and Static Light Scattering

 

 

 

 

Upgrade to plates

Revolutionize your performance by replacing your conventional, cuvette-based dynamic light scattering unit with the automated DynaPro Plate Reader. The DynaPro excels at high-throughput sizing and screening of pharmaceutical and nanoparticle formulations to enable novel workflows that simply would not be possible with other DLS instruments. Even if you need to run just a handful of samples, it's a great productivity booster.

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Walk-up operation with DYNAMICS Touch^™

The DynaPro Plate Reader is the only plate-based DLS instrument with a fully integrated, walk-up measurement experience. DYNAMICS Touch is an intuitive—yet powerful—DLS measurement app for collecting, analyzing, and reporting dynamic and static light scattering data. Via the full-size touch screen display, DYNAMICS Touch guides you through the entire setup and measurement process, expertise not required. It tells you if the data quality is sufficient for confidence in the results and displays them instantly, no matter how many wells you measure. Data files and concise PDF reports can be stored on the instrument or transferred via the network. You can even send the report to your phone!

 

Comprehensive solution

For biotherapeutic development, the DynaPro Plate Reader is a comprehensive solution for stability evaluation. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (T_onset, T_m, T_agg) and colloidal stability (A₂/B₂₂, k_D) may be quantified with plate-based DLS and SLS. Even viscosity of concentrated protein solutions can be screened across dozens of formulations in a single plate.

For nanoparticle formulation and process development, including viral vectors, non-viral vectors and nano-drug delivery vehicles, the DynaPro adds particle concentration / physical titer to basic DLS measurements of size and polydispersity. Its automation is particularly valued to screen fractions during process development and then to quantify CQAs in quality control.

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Compatible with DYNAMICS^™

The DynaPro Plate Reader is operated by DYNAMICS, our powerful software package for collecting, analyzing, and reporting automated batch dynamic and static light scattering data.

 

DYNAMICS is offered with a 21 CFR Part 11 compliant Security Pack option, enabling the use of the DynaPro Plate Reader in a GMP environment.

 

 

 

Revolutionize DLS

The DynaPro Plate Reader offers dynamic light scattering (DLS) and static light scattering (SLS) measurements of the size, molecular weight, concentration and interactions of proteins, nanoparticles and other macromolecules in situ in industry-standard microwell plates. No liquid handling occurs after dispensing into the plates, to maximize throughput and minimize sample carryover. This ground-breaking instrument provides rapid optimization of the formulation process for:

• Biotherapeutics such as monoclonal antibodies and virus-like particles (VLPs)
• Nanoparticle drug delivery vehicles including liposomes and conjugated gold NPs
• Viral and non-viral gene vectors like AAV, adenovirus, lentivirus and LNP

A wealth of applications

The DynaPro Plate Reader provides unparalleled levels of ease-of-use, productivity, reproducibility, and flexibility in order to address additional demanding applications such as:

• Assessing aggregation or oligomerization
• Quantifying AAV titer for multiple processes
• Determining protein stability-indicating parameters such as thermal denaturation and colloidal stability
• Optimizing protein crystallization
• Screening high-concentration protein solutions for reversible association and viscosity

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Key features

• Measure hydrodynamic radius from 0.5 nm to 1000 nm
• Determine particle concentration for particles up to 320 nm in diameter
• Sensitivity for size down to 0.125 mg/mL lysozyme
• Measure weight-average molar mass from 1 to 1000 kDa
• Industry-standard microwell plates of 96, 384 or 1536 wells
• Sample volume from 4 μL to 150 μL
• All measurements made in situ in the wells
• Temperature ramps from 4 °C to 85 °C
• Integrates with plate-based liquid handling robotics

Supported Well Plate Formats	96, 384 or 1536 *
Dynamic Light Scattering
Size Range (Radius, Rh)	0.5 to 1000 nm
Minimum Concentration @ 14 kDa	0.125 mg/mL†
Static Light Scattering
Molar Mass Range	300 Da to 1 MDa††
Minimum Concentration @ 67 kDa	1 mg/mL†††
Optics
Laser Wavelength	830 nm
Laser Power Control	Programmable 10% – 100%
Attenuation Range	1 to 105
Temperature Control	4 to 85 °C ††††
Fluidics
Minimum Sample Volume	4 µL †††††
Data Acquisition Time	1 to 3600 seconds per well per read
Read Time per Well	5 – 20 seconds
Electronics
Correlator	512 channels ††††††
Onboard Camera	3 megapixels †††††††
Digital Communication	Ethernet (TCP/IP)
Dimensions	60 cm (L) x 36 cm (W) x 25 cm (H)

* Supports many industry standard well plates.

^† Minimum concentration specified with 50 µL of Lysozyme (14 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

^†† Upper limit depends on conformation: it is limited to a maximum R_h of 12 nm.

^††† Minimum concentration specified with 50 µL of BSA (67 kDa) sample in a 384 well plate, Wyatt P/N P8803-384, with 100 seconds acquisition.

^†††† Accuracy of ± 0.5 °C for 4 – 50 °C, and ± 1 °C for 50 – 80°C. Minimum temperature of 4 °C requires a laboratory temperature of 24 °C or below.

^{†††††} Minimum volume specified with 2 mg/mL Lysozyme in a 1536 well plate, Wyatt P/N P8803-1536, with 25 seconds acquisition.

^{††††††} 100 nsec sampling time in a multi-tau layout

^{†††††††} Camera operates with sample temperature in the range of 4-85°C.