Lipophilic ORAC Antioxidant Capacity Assay Kit

 

 

 

소개 (Description)

TAC is an important parameter to assess the redox status of biological and food samples. Non-enzymatic antioxidants can be either lipophilic(fat-soluble) or hydrophilic (water-soluble). Most TAC assays are unable to detect the presence of lipophilic antioxidants leading to an under estimation of the real antioxidant powerof a sample.

 

Classical ORAC assay (BQC ORAC Assay) uses aqueous solvents that are not suitable for dissolving lipophilic antioxidants. A modification of the classical protocol has been introduced in the BQC Lipophilic ORAC assay to allow the determination of lipophilic antioxidants. BQC Lipophilic ORAC (Oxygen Radical Antioxidant Capacity) Assay Kit has been designed for the measurement the antioxidant capacity of lipophilic antioxidants in a wide range of samples and organic extracts.

 

 

 

측정 원리 (Assay Principles)

The ORAC Assay is based on the inhibition of fluorescein oxidation byperoxyl radical in the presence of antioxidants. Peroxyl radical (ROO·) isproduced by a free radical initiator (AAPH: 2,2’-azobis-2- methylpropanimidamide dihydrochloride) which produces a fluorescent quenching of fluorescein over time. The presence of antioxidants inhibits the free radical damage to fluorescein, resulting in a preservation of thefluorescent signal.

 

 

 

 

The sample antioxidant capacity correlates to the area under the fluorescence decay curve (AUC). The AUC is used to determine the antioxidant activity in a sample and is compared to an antioxidant standard curve prepared using the water-soluble vitamin E analog Troloxas standard. ORAC results are expressed as Trolox equivalents (TEAC).

 

 

For biological samples and hydrophilic antioxidants (aqueous samples/extracts) 

KF01004-100     Lipophilic ORAC Antioxidant Capacity Assay Kit     100 tests

KF01004-200     Lipophilic ORAC Antioxidant Capacity Assay Kit     200 tests

KF01004-400     Lipophilic ORAC Antioxidant Capacity Assay Kit     400 tests 

For lipophilic antioxidants (organic extracts) 

KF01004B-100     Lipophilic ORAC Antioxidant Capacity Assay Kit     100 tests

KF01004B-200     Lipophilic ORAC Antioxidant Capacity Assay Kit     200 tests

KF01004B-400     Lipophilic ORAC Antioxidant Capacity Assay Kit     400 tests 

 

 

 

 

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