VERO Residual DNA Quantitation Kit  

Accurate, reliable screening for contaminating residual host cell DNA

 

 

This YouSeq kit has been designed to quantitate residual DNA from Vero in cell lines which are used in the production of biopharmaceutical products. This is a key impurity for clearance during the purification process. Regulatory guidance for products produced in cell culture makes it very clear that DNA content in the final product should be minimal.

 

• Vero Residual DNA Quantitation Kit Handbook

 

1.  Kit Contents

 

 • Vero primer/probe set

 • YouSeq Lyophilised TetraTM 2X qPCR MasterMix

 • Positive control/DNA standards

 • MasterMix resuspension buffer

 • RNase/DNase free water

 • Template resuspension buffer

 • ROX passive reference

 

2.  Application

 

To screen for contaminating residual host cell DNA it is critical to use the most sensitive technology possible. Traditional methods have proven laborious and lack sensitivity and specificity. The YouSeq Vero Residual DNA Quantitation kit resolves all of these issues.

 

3.  Technical Specification

 

 • The YouSeq real-time PCR protocol is fast and easy to set up; less than 30 minutes of hands-on time.

 • 45 cycles of qPCR are then performed in around 90 minutes delivering precise, reliable results in less than 2 hours

 • The Vero kit is designed and tested to provide highly specific detection of the cell line DNA species of interest and 

   will not cross-react with any other species.

 

YSL-qP-VERO-100      Vero Residual DNA Quantitation Kit     100 reactions

 

 

 

Vero Residual DNA Quantitation Kit Handbook