Heparan Sulfate Glycan Array

 

 

Heparan sulfate (HS) glycans are sulfated, unbranched, negatively charged saccharide chains. These linear and complex polysaccharides are found in various tissues and serve as critical components of the extracellular matrix. Heparan sulfate proteoglycans (HSPGs) are chains of HS covalently attached to proteins. The biological functions of HSPGs are determined by each HS chain’s length and sulfation pattern, and alterations of these features significantly impact their biological functions.

 

HS and HSPGs are essential components of the human body. HS and HSPGs interact with diverse proteins to maintain physiological homeostasis. The binding partners of HSPG include cytokines, growth factors, immune receptors, and enzymes. In addition, HSPGs can interact with numerous extracellular matrix proteins to form tissue scaffolds. HSPGs can also promote lipid uptake by binding to lipoproteins and lipoprotein lipase. The diverse biological functions of HS and HSPGs have gained significant interest in the scientific community. Identifying and characterizing these complex carbohydrate structures in various biomedical contexts has become a major discipline in glycomics.

 

Although HS and HSPGs are essential in normal physiology, dysregulation of these carbohydrate molecules has been linked to diseases. For example, HSPGs have been implicated in cancer pathogenesis, impacting processes such as tumor-induced angiogenesis, immune evasion, and remodeling of the tumor-associated ECM. In addition, HSPGs have been associated with amyloid diseases such as Alzheimer’s disease, Parkinson’s disease, and TTR amyloidosis due to the occurrence of HSPGs in amyloid plaques and the observed fact that HS chains promote fibrillogenesis. HS and HSPGs are also receptors or co-receptors for invading viruses or other microorganisms to gain entry into the host cell. The roles of HS and HSPGs in disease trajectory are evident, and the underlying molecular mechanisms warrant further investigation. Understanding the biological functions of these molecules in human health and disease is indispensable.

 

ZBiotech has developed a robust microarray platform that allows researchers to explore binding interactions with 24 structurally – defined heparin and heparan sulfate structures varying in length and sulfation. The heparan sulfate (HS) glycan array can be applied to determine the binding specificities of various biological samples, such as proteins, antibodies, cells, vehicles, and viral particles. Each array contains 8 or 16 identical subarrays, enabling the simultaneous analysis of multiple samples. The heparan sulfate glycan array provides high-throughput and reliable glycan-binding information with a simple assay format that only requires a small sample volume. Results can be obtained in only a few hours, making investigating heparin/heparan sulfate binding easy and efficient. The heparan sulfate glycan array can be customized to meet individual client needs. Assay services are available upon request.

 

 

Features

•  24 structurally – defined heparin and heparan sulfate glycan structures

•  Unrivaled sensitivity and specificity

•  Simple assay format

•  Small sample volume

•  Customizable (select HS structures for a specific microarray format)

•  Assay service available

 

 

Applications

•  Evaluate binding specificities of heparin/heparan sulfate-interacting proteins

•  Evaluate binding specificities of heparin/heparan sulfate-interacting antibodies

•  Study virus – heparin/heparan sulfate interactions

•  Study bacteria – heparin/heparan sulfate interactions

•  Study vesicle – heparin/heparan sulfate interactions

•  Study cell – heparin/heparan sulfate interactions

 

 

Antithrombin III binds to specific heparan sulfate glycans

 

The heparan sulfate glycan array was assayed with antithrombin III (10 μg/ml), followed by an anti-antithrombin III antibody (2 μg/ml), and then an anti-mouse IgG antibody (10 μg/ml). The array was scanned with a microarray scanner at 532nm wavelength. Positive control showed bindings. Antithrombin III binds to HS023 and HS024.

 

 

 

List of heparin & heparan sulfate glycan structures on the array (download the PDF)

 

 

 

 

10604-8S         Heparan Sulfate Glycan Array Slide (8 samples)

10604-16S        Heparan Sulfate Glycan Array Slide (16 samples)

10604-8K         Heparan Sulfate Glycan Array *Assay Kit (8 samples)

10604-16K        Heparan Sulfate Glycan Array*Assay Kit (16 samples)

 

* The assay kit contains a microarray slide, a bottle of blocking buffer, a bottle of assay buffer, a hybridization chamber, and sealing films. The hybridization chamber is a device that holds the array slide, assay buffer, and samples to be analyzed. 

 

 

 

List of heparin & heparan sulfate glycan structures on the array (download the PDF)

Protocol & User Manual (download the manual)