Bacterial Glycan Array

 

 

Bacterial cell walls are coated with a dense layer of sugar molecules (polysaccharides). These sugar molecules are essential for bacterial survival as they function as a barrier to protect from environmental stressors. Therefore, the sugar coat is a common target for developing antimicrobial agents.

 

Lipopolysaccharides (LPS) are polysaccharide-based molecules found on the outer membrane of Gram-negative bacteria. Each LPS consists of a lipid and a large polysaccharide core. The polysaccharide core features a repetitive sugar polymer called O-antigen. O-antigen is found on the outer portion of the LPS, is variable in nature, and plays a crucial role in host-pathogen interactions. The host often develops O-antigen-specific immunity against invading bacteria. However, excessive LPS-induced immune responses can have detrimental effects. For example, a profound inflammatory response often induces septic shock manifest with hemodynamic decompensation. Aberrant host responses to the LPS O-antigens also link to diabetes, chronic inflammation, and neurological degeneration. Therefore, uncovering the specific interactions between LPSs and the host immune system could pave new avenues to develop novel therapies for LPS-associated disorders.

 

ZBiotech has developed a robust microarray platform that allows researchers to explore the interactions between bacterial O-antigens and various biological samples. The Multivalent Surface Coating (MSC) technology allows efficient immobilization of bacterial polysaccharides and lipopolysaccharides on a solid microarray surface for high-throughput binding analysis. This array features 26 O-antigens (LPS) purified from multiple bacterial strains. Each array slide contains 8 or 16 identical subarrays, enabling the simultaneous analysis of various samples. It can be used to define LPS-binding specificities of different biological samples, for example, proteins, antibodies, cells, vehicles, viral particles, and sera. The bacterial glycan array provides high-throughput and reliable glycan-binding information with a simple assay format that only requires a small sample volume. Results can be obtained in only a few hours, making investigating bacterial LPS binding easy and efficient. The bacterial glycan array can be customized to meet individual client needs. Assay services are available upon request.

 

 

Features

•  26 O-antigens (LPS) purified from multiple bacterial strains

•  Unrivaled sensitivity and specificity

•  Simple assay format

•  Small sample volume

•  Customizable (select LPS for a specific microarray format)

•  Assay service available

 

 

Applications

•  Evaluate binding specificities of LPS-interacting proteins

•  Evaluate binding specificities of LPS-interacting antibodies

•  Study cell – LPS interactions

 

 

Salmonella typhimurium-specific antibody preferentially binds to the O-antigen from Salmonella enterica (serotype Typhimurium)

 

The bacterial glycan microarray was assayed with a Salmonella typhimurium-specific antibody (1 μg/mL), followed by an anti-mouse IgG (Cy3) antibody. The array was scanned with a microarray scanner at 532nm wavelength. Positive control showed binding signals as expected. Salmonella typhimurium-specific antibody preferentially binds to the O-antigen purified from Salmonella enterica (serotype Typhimurium).

 

 

 

 

List of bacterial glycans on the array (download the PDF)

 

 

No.	ID	Strain
1	EC1	Escherichia coli O111:B4
2	EC2	Escherichia coli O55:B5
3	EC3	Escherichia coli O26:B6
4	EC4	Escherichia coli J5 (Rc mutant)
5	EC5	Escherichia coli F583 (Rd mutant)
6	EC6	Escherichia coli O128:B12
7	EC7	Escherichia coli K-235
8	EC8	Escherichia coli EH100 (Ra mutant)
9	EC9	Escherichia coli O127:B8
10	EC10	Escherichia coli O113
11	EC11	Escherichia coli K12 (Core of E.coli O111:B4)
12	SE1	Salmonella enterica serotype enteritidis
13	SE2	Salmonella enterica serotype minnesota
14	SE3	Salmonella enterica serotype typhimurium
15	SE4	Salmonella enterica serotype minnesota Re 595 (Re mutant)
16	SE5	Salmonella enterica serotype typhimurium SL1181
17	SE6	Salmonella enterica serotype abortus equi
18	ST1	Salmonella typhosa
19	KP1	Klebsilella pneumoniae
20	SM1	Serratia Marcescens
21	PA1	Pseudomonas aeruginose O10
22	CJ1	Campylobacter jejuni Penner O:19
23	PV1	Proteus vulgaris OX19
24	PM1	Proteus mirabilis OXK
25	PG1	P. gingivalis
26	RS1	R. sphaeroides

 

 

 

 

10610-8S         Bacterial Glycan Array Slide (8 samples)

10610-16S        Bacterial Glycan Array Slide (16 samples)

10610-8K         Bacterial Glycan Array *Assay Kit (8 samples)

10610-16K        Bacterial Glycan Array*Assay Kit (16 samples)

 

* The assay kit contains a microarray slide, a bottle of blocking buffer, a bottle of assay buffer, a hybridization chamber, and sealing films. The hybridization chamber is a device that holds the array slide, assay buffer, and samples to be analyzed. 

 

 

 

List of bacterial glycans on the array (download the PDF)

Protocol & User Manual (download the manual)