Superoxide Anion Assay Kit 

제품 소개

The superoxide anion radical (O2• - ) is a reactive oxygen species (ROS) generated by the donation of an electron to oxygen. It is formed as consequence of exposure to UV light, cigarette smoke, pollutants, radiation or oxidases. At normal levels in the organism, O2• - is involved in signal transduction and immune system, but if it accumulates it causes damage to biomolecules and cellular components leading to numerous diseases including cancer, atherosclerosis and diabetes.

BQC Superoxide Anion Assay Kit is a quick, easy, and reproducible assay to quantify superoxide anion in a wide variety of samples.

분석 원리(Assay Principle)

This kit is based on the reaction of O2• - with hydroxylamine hydrochloride which produces the nitrite anion (NO2-). After nitrite reaction with sulfonamide, diazonium salts are formed. These salts react with N-(1-naphthyl) ethylenediamine dihydrochloride to generate a diazonium product with a maximum absorbance at 540 nm. The amount of this product is proportional to the O2• - content in the sample.

KB03048-100        Superoxide Anion Assay Kit        100 test

KB03048-200        Superoxide Anion Assay Kit        200 test

KB03048-400        Superoxide Anion Assay Kit        400 test

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