NADH Oxidase Activity Assay Kit

소개 (Description)

NADH Oxidase (NOX) (EC 1.6.99.3) catalyzes the reduction of various substrates, including oxygen, while oxidizes NADH to NAD + . It has an important role in maintaining the NAD + /NADH balance and protecting the organisms from oxidative stress. NOX is closely related to the immune response and is widely found in animals, plants, microorganisms and cultured cells.

BQC NADH Oxidase Assay Kit is a quick, easy, and reproducible assay to quantify NOX activity in a wide variety of samples.

분석 원리 (Assay Principles)

The assay is based on the oxidation of NADH to NAD + by NOX. This reaction is coupled with the reduction of 2,6-dichlorophenol indigo (DCPIP). Oxidized DCPIP has a blue color with maximum absorbance at 600 nm, while its reduced formed is colorless. Reduction rate of DCPIP is proportional to NOX activity in the sample.

KB03043-200      NADH Oxidase Activity Assay Kit      200 tests

KB03043-400      NADH Oxidase Activity Assay Kit      400 tests 

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