RarePlex® SynaptophysinCTC Panel Kit    

 

Immunofluorescent detection of Synaptophysin (SYP) expression by epithelial CTCs

 

Tumor expression of Synaptophysin in cancer is an indicator of neuro-endocrine differentiation, a prominent mechanism by which tumors become resistant to therapies. The RarePlex 0920-VB Synaptophysin CTC Panel Kit enables immunofluorescent detection of Synaptophysin (SYP) expression by epithelial circulating tumor cells (CTCs).Analysis of CTCs via blood allows for a less-invasive method to identify cancer cells and can prove helpful in prognosis, treatment selection, patient stratification, and monitoring.

 

With the flexibility of one open channel, the RarePlex Synaptophysin CTC Panel Kit expands clinical research of therapeutic resistance by combining SYP with any other marker of your interest. This assay is designed for use with the AccuCyte® Sample Preparation System and the CyteFinder® Instrument for a blood-to-result solution.

 

 

 

 

Analytic validation studies* using spike-in SYP+ and SYP- cancer cell lines as model CTCs (mCTCs) have demonstrated the RarePlex Synaptophysin CTC Panel Kit to be highly specific, sensitive and accurate:

 

  •  SYP biomarker Accuracy of 99%

  •  SYP biomarker Sensitivity of 99%

  •  SYP biomarker Specificity of 100%

 

*Studies were performed using quantitative mean fluorescence intensity measurements of mCTCs

 

The kit contains a nuclear dye; a panel of antibodies and reagents for detection of CD45 (to exclude WBC), Cytokeratin and EpCAM, and Synaptophysin; instructions and protocols for automated staining and scanning; and guidelines for interpreting results.  

 

 

RareCyte RarePlex Synaptophysin CTC Staining Kit, Ventana (SYTOX)     24-1287-002

This 4-channel kit (Version: 0920-VB) detects SYP in CTCs. The kit contains a nuclear dye and antibodies detecting CD45 (to exclude WBC), CK, EpCAM and SYP. Samples are stained by Ventana® DISCOVERY® ULTRA and imaged on CyteFinder®Instrument.

 

RareCyte RarePlex 22Rv1 spike-in CTC Control Slide, 10-Pack      24-1279-000

Contain monolayer of buffy coat and spiked-in 22Rv1 cells. Compatibility: 24-1250-001, 24-1281-001, 24-1282-001, 24-1287-002.