NanoGenerator™  Nanoparticle Synthesis System 

 

나노파티클(LNP) 합성 시스템 

 

 

Nanoparticles, especially liposome and polymer nanoparticles, have shown great potential for biomedical applications in a variety of fields such as pharmaceutical industries like drug delivery, mRNA vaccines, and biosensing because of its excellent properties.

 

Nanoparticle synthesis by microfluidic technology presents advantages over the conventional batch synthesis processes due to its capacity in better uniformity in size and shape. For example, in the drug delivery field, a variety of nanoparticles such as lipid nanoparticles (LNP), liposomes, and PLGA can be synthesized by using NanoGenerator™ nanoparticle synthesis system. The lipid nanoparticles (LNP), liposomes, and PLGAare the most commonly used biodegradable materials for the delivery of both hydrophilic and hydrophobic compounds.  

 

 

 

 

 

 

NanoGenerator™ 나노 파티클 합성 원리 (Principle of Particle Synthesis) 

 

NanoGenerator™ nanoparticle synthesis system employs microfluidic device for controlled and tunable polymer particles’ production. The schematic below illustrates the device with junction in focused-flow geometry designed for particles’ synthesis. Solvent displacement methods are used for nanoparticles synthesis.

 

 

 

 

 

시스템 장점 (System Benefits)

 

 •  Controllable particle size

 •  Low DPI

 •  High encapsulation efficiency

 •  Standard connection. Easy connect to other components

 •  OEM service and module integration available 

 

 

 

 

 

 

 

 

 

 

미세유체 법의 장점: Traditional Batch Method Vs. Microfluidic Method 

 

 

 

 

 

 

 

합성 어플리케이션 (Synthesis System Applications)

 

Drug Delivery (PLGA, Lipid NP & Liposome)

Droplet microfluidics enables useful platforms for drug delivery vehicles and drug molecules as novel functional materials. Because of uniform size, monodisperse size distribution, and desired properties, droplet microfluidics demonstrates promising potential for the production of complex drug systems.

 

Chemical synthesis

Droplet-based microfluidics has become an important method for chemical synthesis. Droplets are able to act as individual reactions, which are free from contamination from outside. Meanwhile, it also offers benefits of high throughput, small reagent volumes, low cost, portability, a high degree of synthetic control, rapid reaction, etc.

 

mRNA Liposome Vaccine

Liposomes and liposome-derived nanovesicles such as archaeosomes and virosomes have become important carrier systems in vaccine development. The liposome-based vaccine delivery systems has advantages in versatility and plasticity.

 

Gel Particle Synthesis

In the last decade, the gel particles (hydrogels, microgels, and nanogels) has been an area of interest for many researchers and industries. Because of high throughput, mono-dispersity of particles, and low cost, droplet-based microfluidic systems have been widely used for synthesizing these hydrogel particles. 

 

 

 

 

NanoGenerator Flex S Demo (0.2-0.5ml per run)

 

 

 

 

 

 

NanoGenerator Flex M Demo (1.0 - 12.0ml per run)

 

 

 

 

 

 

Microfluidic Mixer Chips:

 

PreciGenome offers a variety of microfluidic chips in different materials to meet most of our customers' application requirements for nanoparticles synthesis.

 

Microfluidic mixer chips are usually for nanoparticles synthesis. Three types of materials, including polymers, glass and silicon, are commonly used to fabricate microfluidic chips. Material of the chip is selected depending on the application requirements, including chip design, types of solvent or reagent used for experiment, needs of the application, budget, and fabrication time,etc.

 

 

 

LipidFlex™

 

LipidFlex™ is a 3-component lipid mixture.

 

LipidFlex™ can be directly used to generate liposome for small molecule encapsulation.

 

In addition of different catonic/ionizable lipids, LipidFlex™ can also be used to generate lipid nanoparticles (LNPs) for nucleic acid encapsulation.

 

 Features :

 • Flexible cationic/ionizable lipid ratio

 • Flexible with various N/P ratio

 • Size: 80-120 nmsynthesis

 • PDI: 0.05-0.2

 • Encapsulation Efficiency (E.E.%): >90% (>95-99% w. optimized conditions) 

 

 

 

NanoGenerator™ Flex

 

 •  NanoGenerator controller, 1 pc

 •  Flex-S module for 0.2-1ml output, 1set

 •  Flex-M module for 1-20ml output, 1set

 •  Microfluidic mixer chips, 2 pcs

 •  LipidDemo kit for liposome synthesis test

 

    Part #: PG-SYN-F (with iFlow Touch controller)

 

 

 

NanoGenerator™ Pro

 

 •  Instrument, 1 pc

 •  Reservoir kit,50ml, 4 sets

 •  Microfluidic mixer chips, 2 pcs

 •  Tubing and fittings, 1 set

 •  Demo reagent kit for liposome synthesis

 

     Part #: PG-SYN-P

 

 

NanoGenerator™ brochure

NanoGenerator™ datasheet and application note

Case study: T cell mRNA transfection