SARS-CoV-2 RNA Polymerase (RdRp) Assays for Drug Discovery

 

The COVID-19 pandemic is caused by infection of SARS-CoV-2 or severe acute respiratory syndrome coronavirus 2, a positive-sense single-stranded RNA virus. The RNA dependent RNA polymerase (RdRp) is a key drug target for this deadly virus. The high throughput SARS-CoV-2 RdRp assay is based on detection of the RNA molecules synthesized by the RNA polymerase in the presence of an RNA template and NTPs. The assay can be performed in a 384-well or 96-well plate format for tests of the enzyme activities of the virus RNA polymerase (SARS-CoV-2 RdRp) and high throughput screening of inhibitors. 

 

The gel-based assay is also available for visualization of the RNA product synthesized by RdRp and inhibition by inhibitors. 

 

 

 

 

 

 

 

1. High throughput assay kits

SARS-CoV-2 RNA polymerase assay kit-100 (Catalog No.S2RPA100K)

Includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 xmM NTPs (ATP, UTP, GTP, CTP) and 700 µl of 10 x fluorescence dye. It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format. The assay kit includes all reagents except the enzyme.

 

SARS-CoV-2 RNA polymerase assay kit plus-20 (Catalog No.S2RPA020KE) 

Includes 75 µl of 10 x Buffer,10 µl of 50 x RNA template, 12 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 150 µl of 10 x fluorescence dye and10 µl of 50 x SARS-CoV-2 RdRp.It is for 20 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 10 assays in a 96-well plate format.

 

SARS-CoV-2 RNA polymerase assay kit plus-100 (Catalog No.S2RPA100KE) 

Includes 300 µl of 10 x Buffer, 50 µl of 50 x RNA template, 60 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 700 µl of 10 x fluorescence dye and50 µl of 50 x SARS-CoV-2 RdRp.It is for 100 assays of SARS-CoV-2 RNA polymerase in a 384-well plate format or 50 assays in a 96-well plate format.

 

 

2. Gel-based assay kit

Gel-based SARS-CoV-2 RdRp assay kit (Catalog No.GS2RPA020KE)

Includes all the reagents for 20 samples in gel-based assays of SARS-CoV-2 RdRp activity.It includes 75 µl of 10 x Buffer, 10 µl of 50 x RNA template, 12 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 10 µl of 50 x SARS-CoV-2 RdRp and 130 µl of 5 x Gel loading buffer.

 

 

3. Functional SARS-CoV-2 RdRp enzyme

 

SARS-CoV-2 RdRp complex – for 100 assays (Catalog No. RDRP-100S2) 

Includes 50 µl of 50 x RNA-dependent RNA Polymerase composed of the complex of the nsp12, nsp7 and nsp8 proteins from SARS-CoV-2 virus. It is for 100 assays in a 384-well plate assay format (25 µl reaction) or 50 assays in a 96-well plate format (50 µl reaction). 

 

Catalog No.	Product Name	Size
S2RPA100K	SARS-CoV-2 RNA polymerase assay kit-100	100 assays
S2RPA020KE	SARS-CoV-2 RNA polymerase assay kit plus-20	20 assays
S2RPA100KE	SARS-CoV-2 RNA polymerase assay kit plus-100	100 assays
RDRP-100S2	SARS-CoV-2 RdRp complex – for 100 assays	100 assays