Fluidity One-W Serum

 

Binding affinity measurement in Serum

using microfluidic diffusional sizing (MDS)

 

 

•  Characterization of protein interactions in solution using microfluidic

   diffusional sizing (MDS)

•  Ability to measure in complex backgrounds such as minimally diluted serum

•  No binding artifacts or non-specific binding, no surface-constraints

•  Independent determination of antibody concentration and affinity for a

   comprehensive immune response assessment

•  Easy-to-use interface and consumable management

•  Disposable single use chips and contained waste minimize risk of cross

   contamination

•  Research use only

 

Quantification of protein interactions

Characterization and quantification of antigen-antibody interactions directly in minimally diluted serum

 

Fingerprints of affinity and concentration in real time

Independent determination of antibody affinity and concentration for comprehensive immune response assessments in real time

 

In-solution measurements in complex backgrounds

Elimination of non-specific binding and other binding artifacts for accurate characterization of protein interactions 

 

 

 

We have developed a new instrument that enables comprehensive profiling of the antibody response 

to SARS-CoV-2 on the basis of affinity—a crucial characteristic of protein interactions that 

traditional antibody tests cannot easily quantify. 

 

In-solution affinity profiling directly in minimally diluted serum Ability to independently

determine antibody affinity and concentration 

 

 

 

 

 

Applications

 

Characterize protein interactions in complex backgrounds 

Determine antibody affinity (antibody binding strength) and concentration directly in patient serum for a comprehensive immune response assessment.

 

Measure in complex backgrounds

Characterize protein interactions in biological mixtures such as crude lysates, plasma without surface constraints or binding artifacts. 

 

How does the Fluidity One-W Serum compare to other techniques

Our approach uses in-solution measurements to eliminate the many drawbacks of immobilized assays including non-specific binding and binding artifacts.

As a result, our assays can be run in native complex backgrounds like minimally diluted serum to better predict in-vivo performance. 

 

 

Disposable Microfluidic Chips

 

Single-use injection molded chips for use with the Fluidity One-W.

 

 

The chips are designed to house the microfluidic channels to ensure reliability and reproducibility of results.

The microfluidic chips for the fluidity One-W are sold as part of a consumable pack with reagent cartridges. These packs come in 2 sizes:

 

•  Small – 4 chip boxes (total 96 chips) and one reagent cartridge.

•  Medium – 12 chip boxes (total 288 chips) and 3 reagent cartridges. 

 

 

 

 

 

 

 

Reagent Cartridges

 

Fluidity One disposible microfluidic chips are bundled with sufficient reagents cartridges for the number of chips you order. These cartridges contain everything you need to perform Microfluidic Diffusional Sizing on the Fluidity One–W and Fluidity One-W Serum. 

 

 

 

User Manuals

Brochure

Publication

 

Application Notes

•  Accurate solution phase affinity profiling of a SARS-CoV-2 antibody in serum

•  Measuring the binding affinity of protein-protein interaction in simple and complex backgrounds

•  Affinity of PD-1/PD-L1 interaction—a comparison between SPR, MST, ITC and MDS 

 

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