미세유체 칩 기반 단일 세포 분석 R&D 시스템  

 

Single Cell Analysis R&D System

 

 

 

 

The single cell analysis system is the study platform or tool to isolate bulk cells into selected single cell for the study of genomics, transcriptomics, proteomics and metabolomics at the individual cell level, enabling systematic investigation of cellular heterogeneity in a wide range of cell populations. It offers insights into the composition, dynamics, and regulatory mechanisms of tissues and cell populations in disease or other studies.

 

PreciGenome provides an automated, microfluidic droplet-based platform for single cell research that encapsulates cells and barcoded beads (the encapsulation rate for hydrogel beads can go up to 90%; encapsulation rate for one cell and one bead in a single droplet can reach as high as 9%.

 

The system includes a pressure control unit, optics, illuminations, an integrated high-speed camera for observation, a stage for holding microfluidic chip, and software for parameter optimization and automation. The system is flexible and can switch between automated mode and manual mode. Users can choose to use our standard and patent pending chip design for single-cell research. Users can also choose to design their own chips. 

 

 

주요 특징(Key Features) :

 

•  Automation

•  Easy to use

•  Flexible and open platform

•  Disposable microfluidic chip

•  Portable system

•  Cell size from 1-15um. Up to 60-100um cell with custom chip design

•  Free training available

•  Free system testing/training 

 

 

 

시스템 구성(System components) :

 

•  PG-MFC-8CH, touch screen controller

•  Microfluidic high-speed imaging platform, including manual motion stage, optics, and high-speed camera

•  Integrated software for automation

•  Microfluidic reservoir kit, 15ml, 2sets

•  Reservoir tube racks, 2pcs

•  Tubing & connectors, 1set

•  Liquid flow sensor (optional)

•  PDMS single-cell chips (optional) 

 

 

 

Single Cell Analysis Platform 어플리케이션

 

 

 

DNA/RNA Sequencing by using hydrogel beans

 

Cell and hydrogel bean Isolation by droplets.This figure shows that 90% of droplets contain single hydrogel bead.

 

 

 

DNA/RNA Sequencing by using barcoded polystyrene beads

 

DropSeq example for scRNA seq. This image of collected droplets encapsulating beads in pink color, cells in yellow color. Droplets inside pink rectangle contain both single bead and cell.

 

PG-SC	Precigenome single-cell analysis platform: 2 different packages	The basic components: 1) Microfluidic pressure controller PG-MFC-8CH 2) High speed imaging system (optional); 3) Microfluidic reservoir connector; 4) MIcrofluidic reservoir kits (3 of 2mL or 15mL) 5) Fittings, connectors, and tubings; 6) A single cell analysis chip;
		PG-SC-8-HSV: includes high speed imaging system
		PG-SC-8: no high speed imaging system