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Organ-on-a-Chip 시스템
제품명: Organ-on-a-Chip 시스템
용도: Mimicking human organ functionality, microphysiology and morphology in vitro
메이커: PreciGenome
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소개

 

Organ-on-a-Chip 시스템  

 

Mimicking human organ functionality, microphysiology and morphology in vitro

 

 

Organ-on-a-Chip (OOC) technology provides a novel in vitro platform with a possibility of reproducing physiological functions of in vivo tissue, more accurately than conventional cell-based model systems.

 

The technology opens up great opportunities for next-generation experiments of mimicking human organ functionality, microphysiology and morphology in vitro, replacing traditional animal-based model systems.

 

By realizing different organ functions on a chip, organ-on-a-chip technology is potentially useful for building models of complex diseases. The technology can also be used to study pharmacokentic model when new drugs are being developed. 

 

 

                OOC System Option #1.PNG         Organ on a Chip System.PNG

 

 

 

시스템 장점(Benefits) :

 

•  Controlled shear stress

•  Long duration experiments

•  Automation

•  Controllable flow rates

•  Ready to connect with the incubator

•  Temperature control module is also available to integrate into the system 

 

 

 

 

 

OOC 시스템 옵션 #1 - Multi-Media Culture System 

OOC System Option #1.PNG

 

 

시스템 구성(System Contents): 

  

•  PG-MFC-LT-2CH controller, 1 pc (PG-MFC with touch screen is 

   available to upgrade)

•  Rotary valve, 1to10 ports, 1 pc

•  Reservoir kits (15mL, 50ml or 1.5ml reservoir volume), 5Sets

•  Organ-on-a-chip (OOC) membrane chip, 1pc (optional)

•  Liquid flow sensor (optional)

•  Tubings and Fittings 

 

 

시스템 셋업 예시 - Lung-on-a-Chip 

 

PG-MFC controller has multiple sources of vacuum and pressure. It meets most requirements of organ-on-a-chip applications.

 

In this lung-on-a-chip case, two pressure and one vacuum sources are used which connect to different inlets of a chip. Two pressure lines push different reagents to deliver into the chip to mimic blood flow into lung and exchange chemicals through cell channel bilayer. The vacuum line connects to the side chamber to simulate the breathing process in a lung. 

 

 

Example #1.PNG

 

 

 

 

Example #2.PNG

Example of sine output on vacuum Line, which is used to actuate the membrane between the top and bottom chambers. Meanwhile, the airflow rate is also monitored in realtime to indicate the leakage in the flow. 

 

 

 

 

 

 

 

 

 

OOC 시스템 옵션 #2 - Culture Media Recirculation System 

Organ on a Chip System.PNG

 

 

시스템 구성(System Contents): 

 

•  PG-MFC controller, 1 pc

•  2 positions/6 ports valve, 1 pc

•  Reservoir kits (15mL, 50ml or 1.5ml reservoir volume), 2 sets

•  Organ-on-a-chip (OOC) membrane chip, 1pc (optional)

•  3-way valves, 2 pcs (only for PG-COC-LT2-REC)

•  Liquid flow sensor (optional)

•  Tubings and Fittings 

 

 

시스템 셋업 예시 - Liver-Heart-on-a-Chip

 

In this example, cell lines are used to mimic heart and livers on a microfluidic chip with reagent recirculation.

 

PG-MFC controller is used to provide pressure to pump reagent from reservoir 1 through liver-heart-on-a-chip to reservoir 2 through a 2 positions/6 ports valve. Combining PG-MFC pressure controller with one 2 positions/6 ports valve and two 3-way valves, the system allows flowing buffers in two separate reservoirs back and forth, but still keeps the flow in the microfluidic chip uni-directional. 

 

Example #3.PNG

 

Example of microfluidic recirculation system setup for the organ-on-a-chip application.

Unidirectional reagent flow is able to run through the chip over days. 


 


As showing in the schematics above, in position 1, reagent flows out from reservoir 1, through the 2 positions/6 ports valve, enters the microfluidic chip from its left side, and flows into reservoir 2. In this position, the 3-way valve on the left side connects pressure source to reservoir 1 and the other 3-way valve connects to the atmosphere.

 

In position 2, reagent flows out from reservoir 2, enters the microfluidic chip from its left side, and flows into reservoir 1. In this position, the 3-way valve on the right side connects pressure source to reservoir 2 and the other 3-way valve connects to the atmosphere. In this application, only a single pressure source is needed. 

 


 

Organ-on-a-Chip Crow Flow Membrane Chip

Organ On a Chip_.PNG

 

The cross-flow membrane chips have two in- and outlet ports above and below the membrane. This allows for experiments such as small molecule transfer measurements, on-chip dialysis or cell culture experiments.

 

MOTiF biochips contain two cavities, each comprising an integrated membrane serving as cell culture area. Cells cultured on the membrane are perfusable independently from both the apical or basal side through upward directed (upper perfusion) and downward directed (lower perfusion) channels. 

Produced by injection molding with welded membrane of polyethylene terephthalate.

 

Applications : 

•  Organ-on-a-Chip

•  Cell Culture

•  Plasma/serum Generation


 

 

Organ-on-a-Chip Models

 

Lung-on-a-chip

The lung-on-a-chip is a complex, three-dimensional model of a living, breathing human lung on a microchip. The device is made using human lung and blood vessel cells and it can predict absorption of airborne nanoparticles and mimic the inflammatory response triggered by microbial pathogens. Lung-on-a-chips are being designed in an effort to improve the physiological relevance of existing in vitro alveolar-capillary interface models. Such a multifunctional microdevice can reproduce key structural, functional and mechanical properties of the human alveolar-capillary interface.

 

Brain-on-a-chip

 

Heart-on-a-chip

 

Kidney-on-a-chip

 

Nephron-on-a-chip

 

Vessel-on-a-chip

 

Skin-on-a-chip

Skin-on-a-chip (SoC) are used for testing of topical pharmaceuticals and cosmetics, studying the pathology of skin diseases and inflammation. Also it is to test for the presence of antigens or antibodies which could denote the presence of a pathogen. 

 


주문정보

PG-OOC     Precigenome Organ on a Chip system: 4 different packages

               1) Microfluidic pressure controller (choose model)

               2) Valve (choose model)

               3) Liquid flow senor

               4) Microfluidic reservoir kits (2 of 2mL or 15mL or 100mL)

               5) Fittings, connectors, and tubings;

               6) A standard Organ-on-a-chip 


PG-OOC-8-Rec1        includes PG-MFC-8CH and one 2 positons/6 ports valve 1/SET 

PG-OOC-LT2-Rec1      includes PG-MFC-LT-2CH and one 2 positions/6 ports valve 1/SET 

PG-OOC-8-MRD        includes PG-MFC-8CH and one 10:1 rotary valve 1/SET 

PG-OOC-LT2-MRD      includes PG-MFC-LT-2CH and one 10:1 rotary valve 1/SET