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DMPD Antioxidant Capacity Assay Kit
제품명: DMPD Antioxidant Capacity Assay Kit
용도: Antioxidant Capacity
메이커: Bio Quochem
카달로그: 다운로드


소개

 

DMPD Antioxidant Capacity Assay Kit


 

소개 (Description)Bioquochem-DMPD-assay-kit-450x450.jpg


Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against harmful effect of free radicals, especially oxygen and nitrogen. Antioxidants are substances with antioxidative properties. They are most commonly present in fruits and vegetables. In addittion, other foods such as wine, meat or eggs contain these substances. 

 

 

DMPD 항산화능 측정 (DMPD Antioxidant Capacity Assay)


Bioquochem recommends DMPD assay kit for antioxidant measurements of beverages. This kit measures the antioxidant activity of compounds that are able to transfer hydrogen atoms.When the compound N,N-dimethyl-ρ-phenylenediamine (DMPD) is in the presence of a suitable oxidant solution, a colored radical cation is formed (DMPD•+). Then, antioxidant compounds, which are able to transfer a hydrogen atom to DMPD•+, cause a decoloration of the solution.

 

In our assay a solution of DMPD at an acidic pH, in the presence of a suitable oxidant solution, can form a stable and colored radical cation (DMPD•+) which shows a maximum of absorbance at 553 nm. Then, antioxidant compounds which are able to transfer a hydrogen atom to DMPD•+ quench the color and produce a decoloration of the solution which is proportional to their amount. This reaction is rapid and the end point, which is stable, corresnponds to a measure of the antioxidative efficiency.


 

 

DMPD (uncolored) + Oxidant + H+→ DMPD•+(purple)(λmáx= 553 nm)


DMPD•+(purple)+ AOH → DMPD(uncolored) +AO 

 

 

Scheme 1. Formation of radical DMPD and its reaction with antioxidants (AOH) 

 

DMPD-infography.jpg           Recta-dmpd-2.jpg


주문정보

KF01001-100      DMPD Antioxidant Capacity Assay Kit      100 test

KF01001-200      DMPD Antioxidant Capacity Assay Kit      200 test

KF01001-400      DMPD Antioxidant Capacity Assay Kit      400 test

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