Superoxide dismutase (SOD) Activity Assay Kit

 

 

소개 (Description)

 

Superoxide dismutases (EC 1.15.1.1, SODs) are metalloenzymes that catalyze the conversion (dismutation) of the superoxide anion (O 2 •− ) into molecular oxygen (O 2) and hydrogen peroxide (H 2O 2), playing a key role in the cellular antioxidant defense system.

 

Free radicals like superoxide anion (O 2 •− ) are strongly associated with many pathological processes.

 

SOD is widely found in animals, plants, and microorganisms. In mammals, there are three isoforms of SOD: the cytoplasmic Cu-Zn-SOD (SOD1), the mitochondrial Mn-SOD (SOD2), and the extracellular Cu-Zn-SOD (SOD3).

 

BQC SOD Assay Kit is a very sensitive assay for SOD activity determination that does not require the performance of a standard curve and can be used with multiple biological samples.

 

 

 

 

 

분석 원리 (Assay Principles)

 

BQC SOD Activity Assay Kit is based on the reduction of the water-soluble tetrazolium salt [WST-1, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt] to the water-soluble formazan dye (WST-1 formazan, λ = 450 nm) by superoxide radicals (O 2 •− ).

 

In the presence of SOD, a decrease in the rate of WST-1 formazan generation is produced due to the dismutation of the superoxide radical (O 2 •− ) catalyzed by the enzyme. Since the generation of WST-1 formazan is proportional to the amount of superoxide radical (O 2 •− ), the SOD activity as an inhibition activity can be quantified by measuring the decrease in the formation of this compound at 450 nm.

 

In this kit superoxide radicals (O 2 •− ) are generated by the hypoxanthine/ xanthine oxidase system.

 

 

 

 

KB03011-100       Superoxide dismutase, SOD Activity Assay Kit       100 test 

KB03011-200       Superoxide dismutase, SOD Activity Assay Kit       200 test 

KB03011-400       Superoxide dismutase, SOD Activity Assay Kit       400 test

 

 

 

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