Lipid Peroxidation – LPO Assay Kit

 

 

소개 (Description)

 

Lipid peroxidation is a well-known example of oxidative damage in cell 

membranes, lipoproteins, and other lipid-containing structures. Peroxidative 

modification of unsaturated phospholipids, glycolipids, and cholesterol can occur 

in different reactions. They can be triggered by i) free radical species such as 

oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated 

reduction of hydrogen peroxide or ii) non-radical species such as singlet oxygen, 

ozone, and peroxynitrite generated by the reaction of superoxide with nitric oxide.

 

Malondialdehyde (MDA) and 4-hydroxyalkenals are important toxic byproducts of 

lipid peroxidation. So, the measurement of the amounts of such aldehydes 

corresponds to an index of lipid peroxidation in vitro and in vivo. 

4-Hydroxynonenal(4-HNE) is a major product of the peroxidative decomposition 

of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic,

mutagenic, and genotoxic properties. Furthermore, increased levels of HNE were 

found in plasma and various organs under oxidative stress conditions. In fact, MDA

is in many instances the most abundant individual aldehyde resulting from lipid peroxidation. 

In vitro MDA can alter proteins, DNA, RNA, and many other biomolecules.

 

 

 

 

분석 원리 (Assay Principles)

Bioquochem’s LPO assay kit measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed attack at the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). As a result, this reaction gives a diindolylalkane (chromophore) with maximum absorbance in the region of 580-620 nm.

 

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a high molar extinction coefficient at its maximal absorption wavelength of 586 nm.

 

 

 

Reagent A + MDA → C (λmáx = 586 nm)

Reagent A + HNE → C (λmáx = 586 nm)

Scheme 1. Reactions between aldehydes and indoles 

 

 

 

       

 

 

 

KB03002-100      Lipid Peroxidation – LPO Assay Kit      100 tests

KB03002-200      Lipid Peroxidation – LPO Assay Kit      200 tests

KB03002-400      Lipid Peroxidation – LPO Assay Kit      400 tests 

 

 

 

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