Catalase Activity Assay Kit

 

제품 소개

 

Catalases (EC 1.11.1.6) are antioxidant enzymes that catalyze the conversion of hydrogen peroxide (H 2O 2) to water (H 2O) and molecular oxygen (O 2).

 

Catalase is a crucial antioxidant enzyme that mitigates oxidative stress by destroying H 2O 2. H 2O 2 is an oxidative stress inducer that can cause damage to cell at relatively low concentrations. H 2O 2 is also precursor to certain oxidant radical species (e.g. hydroxyl radical).

 

Catalase exists in almost all aerobic organisms (plants, animals, and microbes).

 

BQC Catalase Activity Assay Kit is a highly sensitive and reproducible assay to measure the catalase activity in biological samples such as plasma, tissue homogenates or cell lysates.

 

 

 

 

분석 원리(Assay Principle) 

 

BQC Catalase Activity Assay Kit is based on the peroxidatic activity of the enzyme. In the presence of MeOH and H 2O 2 the enzyme catalyzes the generation of formaldehyde. Once produced, formaldehyde is detected through its reaction with a specific chromophore. This chromophore undergoes a color change from colorless to purple (λ max 540 nm) upon oxidation by formaldehyde. Catalase activity can be, therefore, determined by measuring the formaldehyde generation at 540 nm.

 

 

 

 

KB03012-100        Catalase Activity Assay Kit        100 test

KB03012-200        Catalase Activity Assay Kit        200 test

KB03012-400        Catalase Activity Assay Kit        400 test

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