BCA Protein Assay Kit         Protein Quantifiaction

 

 

제품 소개

 

The bicinchoninic acid (BCA) assay is a protein quantification technique that was first described by Paul K. Smith in 1985.

 

Proteins are biopolymeric structures composed of amino acids that play many critical roles in the body. Protein is also a vital part of the human diet.Protein quantification assays are therefore fundamental to biological research, clinical diagnosis or food industry.

 

The BCA assay has many advantages over the alternatives (e.g. Lowry, Bradford) including compatibility with a wide variety of detergents, stability of the chromophore and protein-to-protein uniformity.

 

BQC BCA Protein Quantification Assay Kit is a simple test for the quantification of proteins in a wide variety of samples. 

 

 

 

 

 

 

분석 원리(Assay Principle)

 

BQC BCA Protein Quantification Assay Kit is based on the bicinchoninic acid (BCA) assay.

 

This method relies on two chemical reactions. The first is the Biuret reaction, in which Cu 2+ is reduced to Cu + by proteins in an alkaline solution. The second reaction is the chelation of reduced cupper ions by bicinchoninic acid to produce a purple complex with strong absorbance at 562 nm. The protein concentration is a sample is determined from a calibration curve using bovine serum albumin (BSA) as standard. 

 

 

 

 

KB03005-200          BCA Protein Assay Kit         200 test 

KB03005-1000        BCA Protein Assay Kit        1000 test 

Publications