Bradford Protein Quantification Assay    Protein Quantifiaction 

 

 

제품 소개

 

The Bradford Protein Assay is a biochemical assay for determining the total level of protein in a solution, first described by Marion M. Bradford in 1976.

 

Proteins are biopolymeric structures composed of amino acids that play many critical roles in the body. Protein is also a vital part of the human diet. Protein quantification assays are therefore fundamental to biological research, clinical diagnosis or food industry.

 

The BCA assay has many advantages over the alternatives (e.g. Lowry, Bradford) including compatibility with most salts, solvents, buffers, thiols, reducing substances, and metal chelating agents encountered in protein samples.

 

The main disadvantage of this assay is its incompatibility with surfactants at concentrations routinely used to solubilize membrane proteins.

 

BQC Bradford Protein Quantification Assay Kit is a simple and fast test for the quantification of proteins in a wide variety of samples. 

 

 

            

 

 

분석 원리(Assay Principle)

 

BQC Bradford Protein Quantification Assay Kit is based on the Bradford assay. The Bradford protein assay is a dye-binding assay based on the differential color change of a dye in response to protein concentration. In an acidic media, proteins bind to Coomassie Brilliant Blue G-250 dye producing a shift of the dye maximum absorbance wavelength from 465 to 595 nm.

 

The amount of the dye-protein complex formed is directly related to a protein concentration and can be spectrophotometrically estimated by measuring the absorbance at 595 nm. The protein concentration is a sample is determined from a calibration curve using bovine serum albumin (BSA) as standard. 

 

 

 

 

 

KB03003-2000        Bradford Protein Quantification Assay       2000 test 

KB03003-4000        Bradford Protein Quantification Assay       4000 test

 

 

 

Publications