Protein Carbonylation Assay Kit

제품 소개

Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or to protect against irreversible damage that causes the inactive proteins to accumulate or become degraded.

Carbonylation, an irreversible oxidative damage, involves the oxidation of side chains of amino acids to aldehydes or ketones. Lysine, arginine, proline, and threonine sidechains can be oxidatively converted to reactive aldehyde or ketone groups causing inactivation, crosslinking or breakdown of proteins.

Protein Carbonylation can be detected and quantified at the global level in proteins and protein mixtures using derivatization of carbonyl groups with hydrazines followed by spectrophotometric measurement.

BQC Protein Carbonyl Assay Kit is designed to detect carbonylated proteins in a simple and accurate test with high reproducibility. 

분석 원리(Assay Principles)

This Assay Kit is based on the 2,4-dinitrophenylhydrazine (DNPH) method, a widely used method for the determination of protein carbonylation. This method utilizes the reaction of carbonyl groups with DNPH molecule leading to the formation of protein-bound 2,4-dinitrophenylhydrazone with absorbance at 370 nm. Dinitrophenylhydrazone can be detected and quantified spectrophotometrically because it is characterized by a typical absorption spectrum with a maximum at 365–375 nm. 

KB03044-200      Protein Carbonylation Assay Kit      200 test 

KB03044-400      Protein Carbonylation Assay Kit      400 test 

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