Product
소개
Lowry Protein Quantification Assay Kit
제품 소개
The Lowry Protein Assay is a biochemical assay for determining the total level of protein in a solution, first described in 1951. This method has been the most widely used assay to estimate the amount of proteins in biological samples due to its sensitivity, simplicity, and precision.
Proteins are biopolymeric structures composed of amino acids that play many critical roles in the body. Protein is also a vital part of the human diet.Protein quantification assays are therefore fundamental to biological research, clinical diagnosis or food industry.
The Lowry Assay is relatively sensitive but requires more time than other assays and is susceptible to many interfering compounds.
BQC Lowry Protein Quantification Assay Kit is a simple test for the quantification of proteins in in different type of samples.
분석 원리(Assay Principle)
BQC Lowry Protein Quantification Assay Kit is based on the Lowry method.This method relies on two chemical reactions. The first is the Biuret reaction, in which protein reacts with cupric sulfate and tartrate in an alkaline solution, resulting in formation of copper-protein complexes. The second reaction is the reduction of the Folin-Ciocalteu Reagent (phosphomolybdate and phosphotungstate) by the previously formed chelated copper complexes producing a colored product that can be measured at 660 nm. The protein concentration is a sample is determined from a calibration curve using bovine serum albumin (BSA) as standard.
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