DNA Quantitation Kit – Hoechst Assay       

소개 및 원리  

DNA is a reliable endpoint for cell proliferation assays. This proliferation assay, is based on the enhanced fluorescence and shift in the emission wavelength of the fluorochrome Hoechst 33258 upon binding cellular DNA.

At time points of interest, the user have to empty the plates and store them frozen. When the assay is to be performed, cultures are briefly incubated in a lysate media and frozen again. This process lyses the cells and allows rapid and thorough mixing of the fluorochrome and cellular DNA. Freezing permits convenient storage of cultures until the time of assay, then allowing batching experiments. This fact, results in a reduction of the processing time and also gives a better intra and inter-experimental standardization.

The assay here presents, generates a linear standard curve for DNA fluorescence versus cell number. This, enables the rapid and accurate measurement of cell 

number involving minimal processing time, making this assay suited for cell proliferation studies. 

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