MTT Cell Proliferation Assay Kit (cells in suspension)     

 

소개 (Description)

Cell proliferation has been shown to have multiple functions in development and patternformation, including roles in growth, morphogenesis, and gene expression. Methods commonlyused for this purpose are hemocytometer counting, determination of protein content, wet or dryweight measurement, and determination of the optical density (OD). While hemocytometercounting, and protein determination have the disadvantage of being time-consuming andtedious, the measurement of wet or even dry weight is not practical for very small culturevolumes.

 

An alternative method is based on the transformation and colorimetric quantification of MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The respiratory chain and otherelectron transport systems reduce MTT and other tetrazolium salts and thereby form non-watersoluble violet formazan crystals within the cell. The amount of these crystals can be determinedspectrophotometrically and serves as an estimate for the number of mitochondria and hencethe number of living cells in the sample. These features can be taken advantage of in cytotoxicityor cell proliferation assays, which are widely used in immunology, toxicology, and cellular biology.

원리 (Assay Principles)

The principle of the MTT Cell Proliferation Assay is based in mitochondrial activity. For viable cells,mitochondrial activity is constant and thereby an increase or decrease in the number of viablecells is linearly related to mitochondrial activity. The mitochondrial activity of the cells is reflectedby the conversion of the tetrazolium salt MTT into formazan crystals, which can be solubilized forhomogenous measurement. Thus, any increase or decrease in viable cell number can bedetected by measuring formazan concentration spectrophotometrically using a plate reader at 570 nm vs. 690 nm.

 

 

 

 

 

 

KF03001B-500        MTT-B Cell Proliferation Assay Kit (cells in suspension)        500 test

KF03001B-2000       MTT-B Cell Proliferation Assay Kit (cells in suspension)       2000 test

KF03001B-5000       MTT-B Cell Proliferation Assay Kit (cells in suspension)       5000 test

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