Gomori Trichrome Stain Kit

Gomori trichrome stain is used to demonstrate muscle fiber and cytoplasm in red, nuclei in black and collagen containing connective tissue in blue. Weigert’s Hematoxylin and Aniline blue are included to improve staining balance.

 

 

        

 

 

 

사용 및 시료 전처리 (Uses & Sample Preparation) 

Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining. Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.

 

키트 구성 (Kit Components)

•  Hematoxylin Weigerts Reagent A

•  Hematoxylin Weigerts Reagent B

•  Aniline Blue

•  Bouin Fixative

•  Glacial Acetic Acid 0,5%

•  Trichrome stain 

 

염색 과정 (Procedure) 

1.  Bouin fixative at 56 ºC

2.  Allow the solution to cool. Wash with tap water

3.  Mix Hematoxylin Reagent A and B and add to sample

4.  Wash in running tap water

5.  Add Trichrome stain. Solution can be reused if filtered.

6.  Add 0,5% Glacial acetic acid

7.  Dehydrate with graded ethanol, clear and coverslip 

 

 

염색 특징 (Stain Characteristics) 

Nuclei can be visualized as black, muscle fiber and cytoplasm in red and collagen containing connective tissue in blue. 

KH07002-125        Gomori Trichrome Stain Kit        125 ml

KH07002-500        Gomori Trichrome Stain Kit        500 ml

MSDS_KH07002 – Gomori Trichrome Kit_ENG