Periodic Acid Schiff (PAS) – Mayer’s Hematoxylin

Periodic Acid Schiff staining technique is used to demonstrate simple polysaccharides, neutral muco-polisaccharides, mucoproteins, and glicolipids. In this kit, Mayer’s hematoxylin is used to stain cell nuclei.

 

 

 

    

 

 

 

사용 및 시료 준비 (Uses & Sample Preparation)

Designed for research use only, it is not approved for human or animal use, or clinical diagnosis.

키트 구성 (Kit Components)

•  Schiff Reagent

•  Periodic acid 0,5%

•  Sulfurose Acid Reagent

•  Mayer’s Hematoxylin 

 

염색 과정 (Procedure)

1.  Deparaffinize and hydrate

2.  Add periodic acid 0,5%

3.  Rinse with distilled water

4.  Add Schiff Reagent

5.  Rinse with 3 baths of sulfurose acid

6.  Rinse with tap water

7.  Place in hematoxylin

8.  Rinse with tap water

9.  Dehydrate, clear and coverslip

염색 특징 (Staining Characteristics)

Nuclei can be visualized in violet, simple polysaccharides, neutral muco-polisaccharides, glicolipids and basement membrane in red/purple

KH07011-125        Periodic Acid Schiff (PAS) – Mayer’s Hematoxylin        125 ml

KH07011-500        Periodic Acid Schiff (PAS) – Mayer’s Hematoxylin        500 ml

KH07011_Periodic Acid Schiff (PAS) Mayer’s Hematoxilin

MSDS_KH07011 – PAS MAYER’S_ENG