Oil Red O – Lipid Stain Kit

The Oil Red O kit is used to demonstrate adipocites and neutral triglicerides. This is also useful to demonstrate adipogenesis.

Fat cells and neutral fat will be coloured in red and the nuclei in blue.

 

 

 

 

 

사용 및 시료 준비 (Uses & Sample preparation)

This kit can be used in frozen tissue sections, fresh smears or touch preps. It is recommended the use of saline formalin or Baker’s fixative in order to maintain the phospholipids 

 

 

키트 구성 (Kit Components)

•  Mayer’s hematoxylin

•  Absolute propylene glicol

•  85% propylene glicol

•  Oil Red O Reagent 

 

 

염색 과정 (Procedure)

1.  Rinse slides with distilled water

2.  Place in absolute propylene glycol

3.  Place in Oil Red O solution

4.  Place in 85% propylene glycol

5.  Rinse in two changes of distilled water

6.  Place in Hematoxylin

7.  Rinse well with water

8.  Clear and coverslip 

 

염색 특징 (Staining Characteristics)

Fat cells and neutral fat can be coloured in red and the nuclei in blue-purple 

KH07009-125       Oil Red O – Lipid Stain Kit       125 ml

KH07009-500       Oil Red O – Lipid Stain Kit       500 ml

KH07009_Oil Red O kit

MSDS_KH07009 – Oil Red O Kit_ENG