Product
소개
Periodic Acid Schiff (PAS) – Alcian Blue
PAS-Alcian Blue technique is used to differentiate between acidic mucins as sialomucins, sulfomucin and neutral epithelial mucin. After staining, acidic mucins will be coloured blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour.
사용 및 시료 준비 (Uses & Sample preparation)
Slides should be deparaffinized through xylene or substitute and descending grades of alcohol to distilled water prior to staining.
키트 구성 (Kit Components)
• Schiff Reagent
• Periodic acid 0,5%
• Harris Hematoxylin
• Alcian Blue
염색 과정 (Procedure)
1. Place in Alcian Blue
2. Wash in running tap water and distilled water
3. Place in periodic acid 0.5%
4. Rinse with running tap water and distilled water
5. Apply Schiff’s Reagent
6. Rinse with running tap water and distilled water
7. Apply Hematoxylin
8. Rinse with tap water
9. Dehydrate with graded ethanol, clear and cover
염색 특징 (Staining Characteristics)
Acidic mucins can be coloured in blue, neutral in magenta and nuclei in deep blue. Mixtures of acidic and neutral mucins give blue/ purple colour.
주문정보
KH07010-125 Periodic Acid Schiff (PAS) – Alcian Blue 125 ml
KH07010-500 Periodic Acid Schiff (PAS) – Alcian Blue 500 ml
관련자료