Enzyme for Antibody Research

Nowadays, antibodies have become the most popular studied category for their functions in diseases and drugs. Although the research has been improved step by step from chimeric antibodies to human antibodies, there are still many challenges in the area, such as structure characterization and conjugation. Focused on analysis and modification of peptides and glycans, Creative Enzymes offers high quality enzyme products to support the most advanced research on antibodies. 

 

• Full range of products cover IgG proteases and IgG glycosidase

• Highly site- and sequence-specific

• Innovative and unique enzymes

• Short incubation time, 30 min to 2 h

 

 

 

Proteases

The main function of IgG proteases is to digest IgG to generate Fab and Fc fragements for subsequence analysis or modification purposes.

Cat. No.	Target	Features
NATE-1598	IgG	One specific cleavage site below the hinge region. No risk of over digestion. Digestion under physiological conditions, preserving immunoreactivity.
NATE-1599	Mouse IgG2a, IgG3
NATE-1600	All subclasses IgG (human, mouse, rat, goat and sheep)
NATE-1601	Human IgG1

 

We also have immobilized enzymes for IgG and IgM fragmentation.

IgG/IgM Proteases
Cat. No.	Target	Features
NATE-1864	IgG	Mild digestion conditions, high efficiency. Immobilized for ease of separation from cleavage products.
NATE-1865	Mouse IgG1
NATE-1866	IgG / IgM
NATE-1867	IgM

 

 

Glycosidases 

 

The main function of IgG glycosidases is to hydrolyze glycans from the Fc region. 

 

 

Cat. No.	Target	Features
NATE-1603	Complex N-linked glycans in the native IgG Fc region	Reduce the complexity of antibody Modify glycosylation Rapid and mild reactions
NATE-1604	All glycoforms on IgG, including high-mannose
NATE-0603*	High mannose, hybrid, and complex N-glycans

 

 

Characterization

Arginine specific protease digests proteins C-terminal to arginine residues and is used for antibody characterization. 

 

Cat. No.	Target	Applications
NATE-1602	Arg-X motifs in peptides and proteins	Peptide mapping Protein sequence analysis Protein fingerprinting Post-translational modification analysis

 

 

Antibody Labeling and Conjugation

Creative Enzymes's unique technology for covalent conjugation of a label to the Fc domain of the antibody. Besides, enzymes were developed and optmized as labels for antibody labelling.

Cat. No.	Product Name
NATE-0550	Native Horseradish Peroxidase
NATE-0054	Native Calf Alkaline Phosphatase
NATE-0311	Native Aspergillus niger Glucose Oxidase
DIA-189	Native Escherichia coli β-Galactosidase

 

Antibody Conjugation
Service	Target	Conjugation
Label service	IgG	Alexa Fluor® 488 Biotin Deferoxamine (DFO) Azide Activation Enzyme label