Control Phosphoprotein for ELISA

This product is a single phosphorylated protein that can be used as a positive control of

phosphorylation for on-plate detection (ELISA). Mix 5uL of the control phosphoprotein 

with 95uL of the Binding Buffer to immobilize the protein onto a 96-well plate. 

Can be used to confirm that pIMAGO-based detection is working properly or for other 

phosphorylation detection applications. Provided as 200uL solution 

(enough for 40 experiments). Store at 4 degrees Celsius.

It is always difficult to do absolute quantification using different proteins. Various proteins 

may have different binding efficiency to a microplate. However, for relative quantification 

purposes, the amount of control phosphoprotein that we suggest loading (5uL) contains 

approximately 16 pmol of phosphate.

CNTRL-ELISA       Control Phosphoprotein for ELISA (microplate detection)       20 uL