Control Phosphoprotein for ELISA
This product is a single phosphorylated protein that can be used as a positive control of
phosphorylation for on-plate detection (ELISA). Mix 5uL of the control phosphoprotein
with 95uL of the Binding Buffer to immobilize the protein onto a 96-well plate.
Can be used to confirm that pIMAGO-based detection is working properly or for other
phosphorylation detection applications. Provided as 200uL solution
(enough for 40 experiments). Store at 4 degrees Celsius.
It is always difficult to do absolute quantification using different proteins. Various proteins
may have different binding efficiency to a microplate. However, for relative quantification
purposes, the amount of control phosphoprotein that we suggest loading (5uL) contains
approximately 16 pmol of phosphate.
CNTRL-ELISA Control Phosphoprotein for ELISA (microplate detection) 20 uL