Control Phosphoprotein for Western Blot

This product is a single phosphorylated protein that can be used as a positive control of 

phosphorylation for on-membrane detection (Western Blot). The product has already 

been denatured in LDS and DTT and can be immediately loaded onto a gel (load 10uL 

per well). Can be used to confirm that pIMAGO-based detection is working properly or 

for other phosphorylation detection applications. The product contains 100uL of the 

denatured protein (enough for 10 blots). Store at 4 degrees Celsius.

It is always difficult to do absolute quantification using different proteins. Various proteins

may have different transfer efficiency for Western Blot. However, for relative quantification

purposes, the amount of control phosphoprotein that we suggest loading (10uL) contains

approximately 16 pmol of phosphate.  

CNTRL-WB       Control Phosphoprotein for Western Blot (onmembranedetection)       100 uL