Control Phosphoprotein for Western Blot
This product is a single phosphorylated protein that can be used as a positive control of
phosphorylation for on-membrane detection (Western Blot). The product has already
been denatured in LDS and DTT and can be immediately loaded onto a gel (load 10uL
per well). Can be used to confirm that pIMAGO-based detection is working properly or
for other phosphorylation detection applications. The product contains 100uL of the
denatured protein (enough for 10 blots). Store at 4 degrees Celsius.
It is always difficult to do absolute quantification using different proteins. Various proteins
may have different transfer efficiency for Western Blot. However, for relative quantification
purposes, the amount of control phosphoprotein that we suggest loading (10uL) contains
approximately 16 pmol of phosphate.
CNTRL-WB Control Phosphoprotein for Western Blot (onmembranedetection) 100 uL