DNA and Protein Binding Plates

 

 

Specific interactions between biomolecules are studied by using highly charged 96-well plates. 

For instance, negatively charged DNA, RNA or proteins (the first biomolecule) are bound to the highly positively charged plates. After the plates are blocked with the Nucleic Acid Binding Blocker or BSA, a second biomolecule is added. The second biomolecule will also bind to the plate if it interacts with the first biomolecule. The second molecule can be detected by enzymatic assays if it is an enzyme, fluorescence assay if it is DNA or RNA, or ELISA if its antibody is available. If the second molecule is fluorescence or isotope labeled, the labeled signals can be detected. 

We provide highly charged 96-well black or transparent polystyrene plates for various applications.

 

 

 

 

 

 

 

 

 

1.  DNA-binding plates:

The DNA-binding plates are coated with cations that interact with nucleic acids which are anions. Since the binding is based on charge-charge interactions, the plates may also be used for RNA binding. If the bound DNA is a single-strand DNA, the bound DNA can specifically recognize its complementary strand by G-C and A-T base pairing. Therefore, the 96-well DNA binding plates can be potentially used for studies of nucleic acid – nucleic acid interactions or nucleic acid – protein interactions.

 

 

 

96-well DNA–binding Plates (black) (Catalog No: NBP96B) 

Includes 4 plates. The black background of the plate provides a minimum noise level for fluorescence detection of the bound molecules.

 

96-well DNA–binding Plates (transparent) (Catalog No: NBP96T) 

Includes 4 plates. The transparent background of the plate provides an option of detection of the bound molecules based on light absorbance.

 

Nucleic Acid Binding Blocker (Catalog No: NBP96N) 

Includes 1 ml of 200 x concentrated stock solution. The Nucleic Acid Binding Blocker is used to block non-specific interactions between nucleic acids and positively charged surfaces. In a hetero-phase nucleic acid - nucleic acid interaction, after the first nucleic acid binds to the positively charged solid phase, the solid surface was blocked by the blocker so that only the nucleic acid that recognizes the first nucleic acid will bind to the surface when the surface is exposed to various nucleic acids.

 

 

 

 

 

 

2.  Protein-binding plates

The 96-well Protein-binding Plates can be used to study specific protein-protein, protein-nucleic acid or protein-ligand interactions.

 

96-well Anionic Protein-binding Plates (black) (Catalog No: APP96B) 

Includes 4 plates. The Anionic Protein-binding Plates bind negatively charged proteins. The black background of the plate provides a minimum noise level for fluorescence detection of the bound molecules.

 

96-well Anionic Protein-binding Plates (transparent) (Catalog No: APP96T) 

Includes 4 plates. The Anionic Protein-binding Plates bind negatively charged proteins. The transparent background of the plate provides an option of detection of the bound molecules based on light absorbance.

 

96-well Cationic Protein-binding Plates (black) (Catalog No: CPP96B) 

Includes 4 plates. The Cationic Protein-binding Plates bind positively charged proteins. The black background of the plate provides a minimum noise level for fluorescence detection of the bound molecules.

 

 

 

96-well Cationic Protein-binding Plates (transparent) (Catalog No: CPP96T) 

Includes 4 plates. The Cationic Protein-binding Plates bind positively charged proteins. The transparent background of the plate provides an option of detection of the bound molecules based on light absorbance.