Salt or Buffer Exchange Removal

 

Remove small molecules like salts, free enzyme substrates or ligands from a protein or DNA solution

 

 

ProFoldin desalting columns are designed to remove small molecules like salts, free enzyme substrates or ligands from a protein or DNA solution. After desalting, the protein or DNA sample is in a low-salt buffer composed of 10 mM Tris-HCl, pH 7.5. The columns can also be used for buffer exchange of protein or DNA samples to a desired buffer which is used to preequilibrate the column. 

The principle of desalting is size-exclusion chromatography with a molecular cut-off of 5 kDa. The residual salt concentration in the desalted solution is less than 2 % of the original salt concentration. The protein recovery yield is 98 % or higher.

 

 

 

 

 

 

 

 

Micro Desalting Spin Columns Set (Catalog No. MDC050)

Kit components: 50 spin columns.

 

 

 

 

Preparative Desalting Column Set (Catalog No. PDC020)

Kit components: 20 PD-10 columns.