Protein Folding Solutions for Folding Non-Membrane Proteins

96-Well Protein Folding Plate (Catalog No. PFS096)

Kit components: One 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.

The 96-Well Protein Folding Plate provides 96 diversified conditions for protein folding screens. The 96 folding conditions include various pHs, salt concentrations and additives. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5ml of solutions in each well and can be used for multiple experiments of folding various proteins. About 20 micrograms of guanidine hydrochloride-solubilized proteins from inclusion bodies are used for each condition. Once the active protein is identified, preparative folding solutions for particular conditions are available for preparative scale folding.

Protein Folding Solution Kit (Catalog No. PFS well#)

Contains 5 ml inclusion body solubilizer, 40ml 2 x PFS, 4 ml neutralizer.