Concentration assays for NDPs - ADP, UDP, GDP

 

 

 

1.  ADP

 

The MicroMolar ADP Assay is for measurement of ADP concentrations in a sub-micromolar to low micromolar range. Other nucleoside diphosphates (UDP, GDP and CDP) showed very little background. Nucleoside triphosphates (ATP, UTP, GTP and CTP) and monophosphates (AMP, UMP, GMP and CMP) are not detected. The ADP assay is based on fluorescence measurement with emission at 535 nm and excitation at 485 nm. It is a high throughput assay using regular black 96-well or 384-well plates. Micro-cuvettes may also be used for detection of the fluorescence signals. The assay can be used to measure contamination of ADP in ATP samples or monitor hydrolysis of ATP. It can also be used for measurement of kinase activities and other enzyme activities that generate ADP molecules. The ADP assay is compatible with common reaction buffers with magnesium. It is not compatible with samples containing high concentrations of DNA or RNA.

 

 

 

 

 

 

 

MicroMolar ADP Assay kit - 100 assays (Catalog No. MAD100K)

Kit components: 350μl 10 x Buffer, 33μl 100x MAD reagent 1, 33μl 100x Mad reagent 2, 330μl 10 x fluorescence dye, 30μl 1 mM ADP.

 

MicroMolar ADP Assay kit - 500 assays (Catalog No. MAD500K)

Kit components: 1800μl 10 x Buffer, 165μl 100x MAD reagent 1, 165μl 100x Mad reagent 2, 1800μl 10 x fluorescence dye, 100μl 1 mM ADP.

 

 

 

 

 

 

2.  UDP

The MicroMolar UDP Assay is for measurement of UDP concentrations in a sub-micromolar to low micromolar range. Other nucleoside diphosphates (ADP, GDP and CDP) showed very little background. Nucleoside triphosphates (ATP, UTP, GTP and CTP) and monophosphates (AMP, UMP, GMP and CMP) are not detected. The UDP assay is based on fluorescence measurement with emission at 535 nm and excitation at 485 nm. It is a high throughput assay using regular black 96-well or 384-well plates. Micro-cuvettes may also be used for detection of the fluorescence signals. The assay can be used to measure contamination of UDP in UTP samples or monitor hydrolysis of UTP. It can also be used for measurement of enzyme activities that generate UDP molecules. The UDP assay is compatible with common reaction buffers with magnesium. It is not compatible with samples containing high concentrations of DNA or RNA.

 

 

MicroMolar UDP assay kit - 100 assays (Catalog No. MUD100K)

Kit components: 350 μl 10 x Buffer, 33 μl 100x MUD reagent 1, 33 μl 100x MUD reagent 2, 330μl 10 x fluorescence dye, 30 μl 1 mM UDP.

 

MicroMolar UDP assay kit - 500 assays (Catalog No. MUD500K)

Kit components: 1800 μl 10 x Buffer, 165 μl 100x MUD reagent 1, 165 μl 100x MUD reagent 2, 1800 μl 10 x fluorescence dye, 100 μl 1 mM UDP.

 

 

 

 

 

3.  GDP

 

The MicroMolar GDP Assay is for measurement of GDP concentrations in a sub-micromolar to low micromolar range. UDP and CDP showed very little background. ADP will also be detected. Nucleoside triphosphates (ATP, UTP, GTP and CTP) and monophosphates (AMP, UMP, GMP and CMP) are not detected. The GDP assay is based on fluorescence measurement with emission at 535 nm and excitation at 485 nm. It is a high throughput assay using regular black 96-well or 384-well plates. Micro-cuvettes may also be used for detection of the fluorescence signals. The assay can be used to measure contamination of GDP in GTP samples or monitor hydrolysis of GTP. It can also be used for measurement of enzyme activities that generate GDP molecules. The GDP assay is compatible with common reaction buffers with magnesium. It is not compatible with samples containing ADP or high concentrations of DNA or RNA.

 

 

 

 

 

 

MicroMolar GDP assay kit -100 assays (Catalog No. MGD100K)

Kit components: 350 μl 10 x Buffer, 33 μl 100x MGD reagent 1, 33 μl 100x MGD reagent 2, 330μl 10 x fluorescence dye, 30 μl 1 mM GDP.

 

MicroMolar GDP assay kit -500 assays (Catalog No. MGD500K)

Kit components: 1800 μl 10 x Buffer, 165 μl 100x MGD reagent 1, 165 μl 100x MGD reagent 2, 1800 μl 10 x fluorescence dye, 100 μl 1 mM GDP.