Concentration assays for Amino Acids / Histidine / Cysteine

 

 

 

 

 

1.  Amino acid assay

 

The Amino Acid Assay Kit (Catalog No AAK1000) is for measurement of micromolar to millimolar concentrations of amino acids. The assay sensitivity is 0.01 mM for His and Cys and less than 0.5 mM for most amino acids. The assay is based on the principle that amino acids interacts with dye C53 and enhance the fluorescence intensity at 535 nm (excitation at 485nm).

 

 

 

 

 

 

The assay reagent is sufficient for measurement of 1000 samples using 384-well or 96- well plates. It can also be used for measurement of amino acid concentrations using cuvettes. The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), MgCl2(< 5 mM), CaCl2(<5 mM) and phosphate (< 1 mM). The assay is not compatible with Tris buffer or other primary amine buffers. It is not compatible with DTT or EDTA.

 

 

Amino Acid Assay Kit (Catalog No AAK1000)

 

Includes 1 ml of 10 x dye C53. It is for 1000 assays using 384-well plates or 250 assays using 96-well plates. Cuvettes may also be used for the assays.

 

 

 

 

 

2.  Histidine assay

Histidine is an important molecule in biology and pharmaceutical science. Metabolic block of histidine results in increased concentrations of histidine in blood, urine, and cerebrospinal fluid. Disorders of histidine metabolism is related to diseases such as histidinemia and urocanic aciduria. Histidine is a common ingredient of pharmaceutical products. The MicroMolar Histidine Assay Kit (Catalog No HIS200) is designed for measurement of micromolar concentrations of histidine.

 

 

 

 

 

 

The assay is based on increase of fluorescence at 535 nm of the dye C53 in the presence of histidine. The assay kit can be used for measurements histidine concentrations in biological samples, biochemical reactions, pharmaceutical products and environmental water samples. The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), MgCl2 (< 5 mM), CaCl2 (<5 mM), EDTA (< 1 mM) and phosphate (< 1 mM). It is not compatible with thiol compounds such as DTT. For measurement of other amino acids, see the information of Amino Acid Assay Kit (Catalog No AAK1000).

 

 

 

 

MicroMolar Histidine Assay Kit (Catalog No HIS200) 

Provides the reagent for measurement of 200 samples using 96-well plates. Cuvettes may also be used for measurements.

 

 

 

 

 

 

3.  Cysteine Assay

 

Abnormal cysteine levels in vivo are related to cardiovascular disease, Huntington's disease, HIV infection and cancer. Cysteine is a common reducing agent in many in vitro experiments. The MicroMolar Cysteine Assay Kit (Catalog No CYS200) is designed for measurement of micromolar concentrations of cysteine. The assay is based on increase of fluorescence at 535 nm of the dye R53 in the presence of cysteine. The assay kit can be used for measurements cysteine concentrations in biochemical reactions, pharmaceutical products and environmental water samples.

 

 

 

 

 

 

 

 

 

MicroMolar Cysteine Assay Kit (Catalog No CYS200)

 

Provides the reagent for measurement of 200 samples using 96-well plates. Cuvettes or 383-well plates may also be used for measurements.

The assay is compatible with HEPES buffer. It is not compatible with other thiol compounds such as DTT. For measurement of DTT, see the information of MicroMolar DTT Assay Kit (Catalog No: DTT200).