Concentration assays for Oxaliplatin / Penicillin / Polymyxin / Vancomycin

 

 

 

 

 

1.  Oxaliplatin 

 

Oxlaiplatin is one of the platinum-based anti cancer drugs. It is used for treatment of colorectal cancer through its cytotoxic effects by inhibition of DNA synthesis in cells. The MicroMolar Oxaliplatin Assay Kit (Catalog No OPT200) is designed for high throughput measurement of micromolar concentrations of Oxaliplatin. 

The assay is based on the light absorbance at 440nm. The assay kit can be used for assays of oxaliplatin in drug discovery, drug development, pharmaceutical samples and biological samples.

 

 

 

 

 

 

 

 

 

 

MicroMolar Oxaliplatin Assay Kit (Catalog No OPT200) 

Includes 4 ml of Reagent A, 0.080ml of 50 x Reagent B, and 2 ml of 10 x Reagent C. It is for 200 assays using 96-well plates. Cuvettes may also be used for measurements.

 

 

 

 

 

 

2.  Penicillin

 

Penicillin drugs such as Penicillin G and Penicillin V can be hydrolyzed to form penicilloic acid during storage or transportation due to various reasons including the following: (1) the temperature is too high; (2) the solution pH is not optimal for stabilization; or (3) the sample contains lactamase. 

The hydrolyzed penicillin drug molecule is thermodynamically more stable than the non-hydrolyzed one but completely inactive.

 

 

 

 

 

 

 

 

 

 

 

Penicillin Drug Stability Test Kit (Catalog No PST100)

The Penicillin Drug Stability Test Kit (Catalog No PST100) provides a reagent that quickly detects the hydrolyzed form of penicillin drugs including the commonly used Penicillin G and Penicillin V. The reagent, Dye PST, interacts with alpha-amino acids which are the hydrolysis products of penicillin drugs and generates fluorescence at 535 nm with excitation wavelength at 485nm. The background signal with the non-hydrolyzed penicillin drugs is very low. The kit can be used to detect hydrolysis of penicillin drugs. It can also be used to detect the lactamase activity using penicillin as a substrate.

 

 

 

 

 

 

 

3.  Polymyxin

 

Polymyxins are polypeptide antibiotics that bind lipopolysaccharide (LPS) and disrupt bacterial membranes. They are used for the treatment of Gram-negative bacterial infections. Since polymyxins increase permeability of the bacterial membrane system, they are also used for enhanced release of secreted toxins from bacteria. The MicroGram Polymyxin Assay Kit (Catalog No MPX200) is designed for measurement of microgram/ ml concentrations of polymyxins. 

The assay is based on increase of fluorescence at 535 nm of the assay reagent in the presence of polymyxins. The assay kit can be used for measurement of polymyxin concentrations in drug discovery, drug development and pharmaceutical samples.

 

 

 

 

 

 

 

 

 

MicroMolar Polymyxin Assay Kit (Catalog No MPX200)

Provides the reagent for measurement of 200 samples using 96-well plates. Cuvettes may also be used for measurements.

 

 

 

 

 

 

 

4.  Vancomycin

 

Vancomycin is a glycopeptide antibiotic that blocks the cell wall synthesis of bacteria. The MicroGram Vancomycin Assay Kit is designed for measurement of low micrograms of vancomycin. 

The assay is based on increase of fluorescence at 470 nm of the assay reagent in the presence of vancomycin. The assay kit can be used for measurement of vancomycin concentrations in drug discovery, drug development and pharmaceutical samples. The assay is not compatible with biological samples containing amino acids or other molecules or buffers with amines.

 

 

 

 

 

 

 

 

 

MicroGram Vancomycin Assay Kit (Catalog No VAN100)

Includes 0.5 ml of 10 x VAN dye, 5.5 ml of Assay buffer, 5 ml of reagent A. It is for measurement of 100 samples using 96-well plates. Cuvettes may also be used for measurements.