Concentration assays for EDTA / DTT

 

 

 

 

1.  EDTA

 

EDTA (Ethylenediaminetetraacetic acid) is a common chelating agent in biochemistry. EDTA should be avoided in protein purification with a Ni-column. Free EDTA should not be included in many enzyme reactions where divalent cations such magnesium, calcium and zinc are required for the enzyme activity. The MicroMolar EDTA Assay Kit (Catalog No EDTA200) is designed for measurement of micromolar concentrations of EDTA. 

The assay is based on increase of fluorescence at 535 nm of the dye C56 in the presence of EDTA. The assay kit can be used for measurements EDTA concentrations in biological samples, biochemical reactions and environmental water samples. The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), Tris-HCl (<10 mM), and phosphate (< 1 mM). It is not compatible with thiol compounds such as DTT, 2-mercaptoethanol or cysteine.

 

 

 

 

 

 

 

MicroMolar EDTA Assay kit (Catalog No. EDTA200)

Kit components: 200 ul 100x C56 dye, 1 ml 1 mM EDTA.

 

 

 

 

 

 

2.  DTT

 

DTT (Dithiothreitol) is a common reducing agent in biochemistry. Removal of DTT is required for Cys-based protein labeling and for disulfide bond formation in proteins. The MicroMolar DTT Assay Kit (Catalog No DTT200) is designed for measurement of micromolar concentrations of DTT. 

The assay is based on increase of fluorescence at 535 nm of the dye C55 in the presence of DTT. The assay kit can be used for measurements DTT concentrations in biological samples, biochemical reactions and environmental water samples. The assay is compatible with HEPES buffer, low concentrations of non-ionic detergent (<0.01%), MgCl2 (< 5 mM), CaCl2 (<5 mM), Tris-HCl (<10 mM), EDTA (< 1mM) and phosphate (< 1 mM). It is not compatible with thiol compounds such as 2-mercaptoethanol or cysteine.

 

 

 

 

 

 

 

 

MicroMolar DTT Assay kit (Catalog No. DTT200)

Kit components: 5 ml Dye C55.