Concentration assays for Detergent CMC / Lipids

 

 

 

1.  Detergent CMC

 

The critical micelle concentration (CMC) value of a detergent depends on the solution compositions such as salt concentrations. Knowing the CMC value of the detergent in a particular buffer is essential in membrane protein extraction, purification and crystallization. Membrane proteins tend to aggregate when the detergent concentration is below the CMC. A too high detergent concentration may cause many problems including protein instability, difficulty in purification and crystallization. A low concentration of a detergent may be used for enzyme assays where formation of detergent micelles should be avoided in testing enzyme inhibitors. 

The CMC assay kit is based on the principle that the detergent interacts with the fluorescence dye and enhances the fluorescence intensity at 465 nm (excitation at 360 nm). The kit can be used for measurement of the CMC values of known detergents in solutions with different salt concentrations that affect the CMC values. It can also be used to measure the CMC values of new detergents or to test if a new molecule is a detergent that should have a CMC value.

 

 

 

 

 

 

 

 

 

Detergent Critical Micelle Concentration (CMC) Assay Kit (Catalog No. CMC1000)

 

Kit components: 105μl 1000 x CMC dye.

 

 

 

 

 

2.  Lipids

 

Lipids are essential components of cell membranes. Synthetic lipids or lipids isolated from the nature are used for constructions of bilayer membranes for various applications such as membrane protein reconstitution and liposomal drug formulations.

 

 

 

 

 

 

 

MicroGram Lipid Assay Kit (Catalog number LIP1000)

 

Designed for measurement of various lipids at concentrations of micrograms per milliliter. The assay is based on measurement of fluorescence at 465 nm (excitation at 360 nm). It can be used to measure concentrations of various purified lipids or lipid mixtures such as cell membrane lipids or liposomes.

 

 

 

 

MicroGram Lipid Assay Kit (Catalog number LIP1000)

 

Includes 0.1 ml of 1000 x LIP 10 Dye. It is for measurement of 1000 samples using 96-well plates. Cuvettes may also be used for measurements.