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SELECTRAZYME β-Glucuronidase
제품명: SELECTRAZYME β-Glucuronidase
용도: β-Glucuronidase
메이커: UCT
카달로그: 다운로드


소개

 

 

SELECTRAZYME β-Glucuronidase 

 

 

SelectraZyme.PNG

 

 

Abalone derived β-glucuronidase is used for the enzymatic hydrolysis of glucuronides from urine, blood and serum prior to analysis by enzyme immunoassay, mass spectrometry, high performance liquid chromatography, and other means. Typically, between 1 to 10 units of glucuronidase is used per microliter of the sample matrix. The exact amount needed will depend on the specific conditions used and must be determined empirically.


Abalone derived β-glucuronidase is a crude solution of enzymes. Many β-glucuronidases derived from mollusks also contain sulfatase activity. For this reason, the sulfatase activity of the material is also determined. Abalone derived β-glucuronidase is more thermal tolerant as compared to enzymes derived from E. coli, H. pomatia and bovine liver. Therefore the hydrolysis reaction can be carried out at a higher temperature providing hydrolysis in less time and achieving a higher degree of hydrolysis of metabolites like morphine-3-glucuronide.


Another application of Abalone derived β-glucuronidase is for the deconjugation of steroids in human and animal urine samples. When compared with H. pomatia, Limpet and bovine liver, abalone derived β-glucuronidase provides best results. For reference to this study, please site "Enzymatic Hydrolysis of Conjugated Steroid Metabolites: Search for Optimum Conditions Using Response Surface Methodology" V. Ferchaud, P. Courcoux, B. LeBizec, F. Monteau & F. Andre, The Analyst, 2000, 125, 2255-2259.

 


주문정보

Part Number               Description                                                                      Size          Units



BETA-GLUC-10



Beta Glucuronidase Enzyme (abalone) - liquid form 



10 mL


1



BETA-GLUC-25



Beta Glucuronidase Enzyme (abalone) liquid form 



25 mL


1



BETA-GLUC-50



Beta Glucuronidase Enzyme (abalone) liquid form 



50 mL


1



BETA-GLUC-100



Beta Glucuronidase Enzyme (abalone) liquid form 



100 mL


1



BETA-GLUC-250KU



Beta-Glucuronidase Enzyme (abalone) lyophilized

250,000 fishman units 



250,000 units


1



BETA-GLUC-500KU



Beta-Glucuronidase Enzyme (abalone) lyophilized

500,000 fishman units 



500,000 units


1



BETA-GLUC-1MU



Beta-Glucuronidase Enzyme (abalone) lyophilized

1,000,000 fishman units 



1,000,000 units


1



BETA-GLUC-2MU



Beta-Glucuronidase Enzyme (abalone) lyophilized

2,000,000 fishman units 



2,000,000 units


1
  

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