GlycoLite™ 2200, Evanescent-field Fluorescence Excitation Glycan Profiler

 

Glycan Profiling Analysis Tools using Lectin Microarrays

 

 

GlycoLite™ 2200 is a low cost version of GlycoStation™ Reader 1200 

based on the same principle of evanescent-field fluorescence excitation 

to scan LecChip Ver.1.0.

   

 

GlycoStation™ is a Gold Standard in Glycan Profiling   

  

Applications 

 

•  Biomaker Screening (focusing on glycan structure changes)   

•  Stem Cell Characterization   

•  Quick Monitoring of Therapeutic Protein Glycan Structures  

•  Quick and Easy Glycan Profiling of Infectious Virus and/or Intestinal Bacteria 

 

 

Technical Reports (Notes) 

•   Differential glycan profiling of cell lysates between CHO and Lec1.   

•   Glycan profiling of secreted proteins from cancer cells into culture media    

•   Glycan profiling of living cells    

•   Differential glycan profiling of commercial EPOs   

  

References

 

Altered sialylation induced by radiation exposure

 D. Iizuka, S. Izumi, F. Suzuki, and K. Kamiya, Analysis of a lecin microarray identifies altered sialylation of mouse serum glycoproteins induced by whole-body radiation exposure, J. of Radiation Research, rry100, https://doi.org/10.1093/jrr/rry100.

Major Depressive Disorder

 H. Yamagata, S. Uchida, K. Matsuo, K. Harada, A. Kobayashi, M. Nakashima, F. Higuchi, T. Watanuki, T. Matsubara, and Y. Watanabe, Altered plasma protein glycosylation in a mouse model of depression and in patients with major depression, J. of Affective Disorders, June 2018, Vol.233, pp-79-85, https://doi.org/10.1016/j.jad.2017.08.057.

Exosomes Glycan Profiling Analysis

A. Shimoda, Y. Tahara, S. Sawada, Y. Sasaki, and K. Akiyoshi, Glycan profiling analysis using evanescent-field fluorescence-assisted lectin array: Importance of sugar recognition for cellular uptake of exosomes from mesenchymal stem cells., BBRC, 2017 Sept. 23, 491(3)pp701-707. doi:10.1016/j.bbrc.2017.07126.

Tissue Glycan Mapping

 X. Zou, M. Yoshida, C. Nagai-Okatani, J. Iwaki, B. Tan, K. Hagiwara, T.Sato, Y. Itakura, E.Noro, H. Kaji, M. Toyoda, Y. Zhang, H. Narimatsu, and A. Kuno, A stadardized method for lectin microarray-based tissue glycome mapping, Sci. Rep., 2017 Mar 6;7:43560. doi: 10.1038/srep43560.

Colorectal cancer recurrence and ABA

K. Nakajima, M. Inomata, H. Iha, T. Endoh, N. Shiraishi, K. Kashima, and S. Kitano, Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis, Cancer Med., 2015 (Feb); 4(2): 293-302. doi: 10.1002/cam4.342.

Gastric cancer recurrence and BPL

T. Futsukaichi, T. Etoh, K. Nakajima, T. Daa, H. Shiroshita, N. Shiraishi, S. Kitano, and M. Inomata, Decreased expression of Bauhinia purpurea lectin is a predictor of gastric cancer recurrence, Surg Today, 2015 Oct; 45(10): 1299-306. doi: 10.1007/s00595-015-1127-1.

Fucosylation and Intraductal papillary mucinous neoplasms

K. Watanabe, M. Ohta, K. Yoda, Y. Komori, Y. Iwashita, K. Kashima, and M. Inomata, Fucosylation is associated with the malignant transformation of intraductal papillary mucinous neoplasms: a lectin microarray-based study, Surg Today, 2016 Oct; 46(10): 1217-23. doi: 10.1007/s00595-015-1299-8.

Innate immune responses and Glycosylation

M. Yamamoto-Hino, M. Muraoka, S. Kondo, R. Ueda, H. Okano, and S. Goto, Dynamic regulation of innate immune responses in Drosophia by Senju-mediated glycosylation, Proc. Natl Acad Sci USA, 2015 May 5; 112(18): 5809-14. doi:10.1073/pnas.1424514112.

Biobetter Characterization

M. Roucka, K.Zimmermann, M. Fido, and A. Nechansky, Application of Lectin Array Technology for Biobetter Characterization: Its Correlation with FcyRIII Binding and ADCC, Microarrays 2017, 6(1); doi:10.3390/microarrays6010001.

Muchin-Like Glycopeptides

G. Artigas, H. Hinou, F. G. Martin, H. J. Gabius, and S. Nishimura, Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays, Chem. An Asian Journal, DOI: 10:1002/asia.201601420.

FDA Paper for The use of lectin microarray for assesing glycosylation of therapeutic proteins

L. Zhang, S. Luo, and B. Zhang, mAbs (2016), DOI: 10.1080/19420862.2016.1149662.

FDA Paper for Glycan analysis of therapeutic glycoproteins

L. Zhang, S. Luo, and B. Zhang, mAbs (2015), DOI: 10.1080/19420862.2015.1117719.

Cancer and O-Linked Glycosylation of Muc1

A. Matsuda, A. Kuno, T. Nakagawa, Y. Ikehara, T. Irimura, M. Yamamoto, Y. Nakamura, E. Miyoshi, S. Nakamori, H. Nakanishi, C. Viwatthanasittiphong, P. Srivatanakul, M. Miwa, J. Shoda, and H. Narimatsu, Lectin Microarray-Based Sero-Biomarker Verfication Targeting Aberrant O-Linked Glycosylation on Mucin 1, Anal Chem, 2015 Jul 21; 87(14):7274-81. doi: 10.1021/acs.analchem.5b01329. Epub 2015 Jul 7.

Lymph node metastasis of advanced gastric cacer

K. Yamashita, A. Kuno, A. Matsuda, Y. Ikehara, N. Katada, J. Hirabayashi, H. Narimatsu, M. Watanabe, Lectin Microarray technology identifies specific lectins related to lymph node metastasis of advanced gastric cancer, Gastric Cancer, 2015 Apr.4, PMID: 25840959

Development of lectin microarray

J. Hirabayashi, Synthesiology - English edition Vol.7, No.2, p.105 (Sep. 2014)

Profiling of Cell Surface Glycome of Fungi

A. Shibazaki and T. Gonoi, Lectin microarray technique for glycomic profiling of fungal cell surfaces, Methods Mol Biol., 2014; 1200: 1200-94.

Cancer Diagnosis

H. Narimatsu, Synthesiology - English edition Vol.5, No.3, p.201 (Dec. 2012)

Cancer-specific Glycosylated Podoplanin

Y. Kato and M. K. Kaneko, Scientific reports 4, Article Number: 5924, doi:10.1038/srep05924

Glycan Profiling of Choriocarcinoma

Y. Kobayashi, K. Masuda, K. Banno, N. Kobayashi, K. Umene, Y. Nogami, K. Tsuji, A. Ueki, H. nomura, K. Sato, E. Tominaga, T. Shimizu, H. Saya, and D. Aoki, Glycan profiling of gestational choriocarcinoma using a lectin microarray.

Novel Glycan Marker for Diabetic Nephropathy

K. Inoue, J. Wada, J. Eguchi, A. Nakatsuka, S. Teshigawara, K. Murakami, D. Ogawa, T. Terami, A. Katayama, A. Tone, I. Iseda, K. Hida, M. Yamada, T. Ogawa, and H. Makino, Urinary Fetuin-A is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identifies by Lectin Microarray.

Lectin Microarrays and glycodiagnostic agent

A. Kuno, T. Sato, H. Shimazaki, S. Unno, K. Saitou, K. Kiyohara, M. Sogabe, C. Tsuruno, Y. Takehama, Y. Ikehara, and H. Narimatsu, Reconstruction of a robust glycodiagnostic agent supported by multiple lectin-assisted glycan profiling, Proteomics Clin Appl., 2013 May3, doi: 10.1002/prca.201300010.

Cancer Marker for Cholangiocarcinoma

A. Matsuda, A. Kuno, H. Matsuzaki, T. Kawamoto, T. Shikanai, Y. Nakanuma, M. Yamamoto, N. Ohkohchi, Y. Ikehara, J. Shoda, J. Hirabayashi, and H. Narimatsu, Glycoproteomics-based cancer marker discovery adopting dual enrichment with WFA for high specific glyco-diagnosis of cholangiocarcinoma, J. of Proteomics 85 (2013) p.1-11.

Cancer Marker for Hepatocellular Carcinoma

H. Kaji, M. Ocho, A. Togayachi, A. Kuno, M. Sogabe, T. Ohkura, H. Nozaki, T. Angata, Y. Chiba, H. Ozaki, J, Hirabayashi, Y. Tanaka, M. Mizokami, Y. Ikehara, and H. Narimatsu, J. of Proteome Res., 2013, 12 p.2630-2640. 

Glycan Profiling of Retinal Degradation

Lectin Microarray Profiling and Relative Quantification of Glycome Associated With Proteins of Neonatal wt and rd1 Mice Retinae, S. Ahuja, Invest Ophathalmol Vis Sci. 2013 May 7; 54(5): 3272-80. doi: 10.1167/iovs.12-11363. 

Xenotransplant from pig to human

A lectin array analysis for wild-type and α-Gal-knockout pig islets versus healthy human islets, S. Miyagawa, A. Maeda, S. Takeishi, T. Ueno, N. Usui, S. Matsumoto, T. Okitsu, M. Goto, and H. nagashima, Surg Today, 2013 Apr.3. 

Adult T-cell Leukemia

Glycan Profiling of Adult T-Cell Leukemia (ATL) Cells with the High Resolution Lectin Microarrays, H. Iha and M. Yamada, "T-Cell Leukemia - Characteristics, Treatment and Prevention", book edited by Mariko Tomita, ISBN 978-953-51-0996-9, Published: February 20, 2013 under CC BY 3.0 license, DOI: 10.5772/55386. 

Lectin Microarrays

 Lectin Microarrays: concept, principle and applications, J. Hirabayashi, M. Yamada, A. Kuno and H. Tateno, Chemical Society Reviews, 2013, doi:10.1039/C3CS35419A. 

Liver Fibrosis and Glyco-biomarker

A serum "sweet-doughnut" protein facilitaes fibrosis evaluation and therapy assessment in patients with viral hepatitis, A. Kuno, Y. Ikehara, Y. Tanaka, K. Ito, A Matsuda, S. Sekiya, S. Hige, M. Sakamoto, M. Kaga, M. Mizokami and H. Narimatsu, Scientific Reports 3, Article number: 1065, doi:10.1038/srep01065. 

Dynamic glycome shift on iPS 

Structural and quantitative evidence for dynamic glycome shift on production of induced pluripotent stem cells, K. Hasehira, H. Tateno, Y. Onuma, Y. Ito, M. Asashima, and J. Hirabayashi, Mol Cell Proteomics., 2012 Dec;11(12):1913-23. doi: 10.1074/mcp.M112.020586. 

Endometrial cancers 

Glycan profiling of endometrial cancers using lectin microarray, Y. Nishijima, M. Toyoda, M. Yamazaki-Inoue, T. Sugiyama, M. Miyazawa, T. Muramatsu, K. Nakamura, H. Narimatsu, A. Umezawa, and M. Mikami, Genes Cells, 2012 Oct. 17(10):826-36. doi: 10.1111/gtc. 12003. 

Metastasis-associated changes in glycosylation

Lectin array-based strategies for identifying metastasis-associated changes in glycosylation, S. Fry, B. Afrough, A. Leathem, and M. Dwek, Methods Mol Biol., 2012;878:276-72 

 

Barrett's esophagus 

Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus, Bird-Lieberman EL, Neves AA, Lao-Sirieix P, O'Donovan M, Novelli M, Lovat LB, Eng WS, Mahal LK, Brindle KM, Fitzgerald RC., Nature Med. 2012 Jan 15. doi: 10.1038/nm.2616.