Magic™ Membrane Protein Characterization

Creative Biolabs provides professional custom services in membrane protein characterization to facilitate our customers' research, development, and applications of membrane proteins. Our versatile platform is compatible with various structure, kinetics and function profiling of membrane proteins. This service can be offered separately or as an integral step of our membrane protein preparation package, to ensure that the final product can meet custom standards of purity, function, structure, binding behaviors, etc.

 

Our membrane protein characterization services including but not limited to:

 

 •   Native polyacrylamide gel electrophoresis (PAGE)

 •   SDS polyacrylamide gel electrophoresis (PAGE)

 •   Fluorescence spectroscopy

 •   Circular dichroism spectroscopy

 •   UV-Vis absorption spectroscopy

 •   Chemiluminescence

 •   Radiometry

 •   Dynamic light scattering (DLS)

 •   Analytical gel-filtration/size-exclusion chromatography

 •   Analytical ultracentrifugation

 •   Western blotting

 •   Enzyme-linked immunosorbent assay (ELISA)

 •   Immunoprecipitation

 •   Surface plasmon resonance (SPR) and bio-layer interferometry (BLI)

 •   X-ray crystallography of membrane proteins

 •   Nuclear magnetic resonance (NMR) spectroscopy

 •   Cryo-electron microscopy (Cryo-EM) of membrane proteins

 •   SAXS studies

 •   Function validation

From these services, our customers can obtain information for membrane protein structure, function, activity as follows:

 •   Purity and composition

 •   Topology, size, and oligomeric state

 •   Atomic structure

 •   Thermodynamic and kinetic parameters for binding with drugs, antibodies, and ligands

 •   Thermodynamic and kinetic parameters for folding and oligomerization

 •   Enzyme activity

 •   Signal transduction efficiency and selectivity

 •   Molecule and ion transportation efficiency and selectivity 

Related Sections

Services

• Magic™ Cell-based Membrane Protein Expression
• Magic™ Cell-free Membrane Protein Expression
• Magic™ Membrane Protein Solubilization and Stabilization
• Magic™ Membrane Protein Purification
• Virus-Like Particles (VLPs)