pIMAGO - Western Blot

Universal, Non-Antibody Detection of Protein Phosphorylation

 

pIMAGO is based on water-soluble, globular nanopolymers (i.e., dendrimers) that 

are multi-functionalized with reactive groups (e.g., Ti(IV) ions) for the highly 

specific recognition of phosphate groups, and with ‘reporting’ groups (e.g., 

biotin, fluorophore) that facilitate their detection. 

Multiple functionalized groups per dendrimer ensure very strong binding to the 

phosphoproteins (resulting in high specificity) and significant signal amplification 

from many “reporting” groups (resulting in high sensitivity).

 

 

pIMAGO - Western Blot 분석 과정  

 

 

 pIMAGO-biotin Phosphoprotein Detection on Membrane Kits 

 

All kits include pIMAGO-biotin reagent, streptavidin-based detection reagent, blocking buffer, pIMAGO buffer, 

washing buffer, and a control phosphoprotein. ECL substrate is not included.

 

800-10      pIMAGO HRP Phosphoprotein Detectionon Western Blot (complete kit)        10 mini-blots

800-40      pIMAGO HRP Phosphoprotein Detectionon Western Blot (complete kit)        40 mini-blots

801-10      pIMAGO Fluor 550 Phosphoprotein Detectionon Western Blot (complete kit)        10 mini-blots

801-40      pIMAGO Fluor 550 Phosphoprotein Detectionon Western Blot (complete kit)        40 mini-blots

802-10      pIMAGO Fluor 680 Phosphoprotein Detectionon Western Blot (complete kit)        10 mini-blots 

802-40      pIMAGO Fluor 680 Phosphoprotein Detectionon Western Blot (complete kit)        40 mini-blots

803-10      pIMAGO Fluor 800 Phosphoprotein Detectionon Western Blot (complete kit)        10 mini-blots

803-40      pIMAGO Fluor 800 Phosphoprotein Detectionon Western Blot (complete kit)        40 mini-blots

Protocols 

•  pIMAGO-biotin phosphoprotein detection on Western Blot – Protocol

 

Technical Documents 

•  pIMAGO-biotin HRP detection on Western Blot – 10 blots – Technical Document (Cat# 800-10)

•  pIMAGO-biotin HRP detection on Western Blot – 40 blots – Technical Document (Cat# 800-40) 

•  pIMAGO-biotin Fluor 550 detection on Western Blot – 10 blots – Technical Document (Cat# 801-10) 

•  pIMAGO-biotin Fluor 550 detection on Western Blot – 40 blots – Technical Document (Cat# 801-40)

•  pIMAGO-biotin Fluor 680 detection on Western Blot – 10 blots – Technical Document (Cat# 802-10) 

•  pIMAGO biotin Fluor 680 detection on Western Blot – 40 blots – Technical Document (Cat# 802-40) 

•  pIMAGO-biotin Fluor 800 detection on Western Blot – 10 blots – Technical Document (Cat# 803-10)

•  pIMAGO biotin Fluor 800 detection on Western Blot – 40 blots – Technical Document (Cat# 803-40)

 

MSDS

•  pIMAGO Kit MSDS