pIMAGO - Detection on Microplate

Non-Antibody Method for Universal Detection of Protein Phosphorylation

pIMAGO is based on water-soluble, globular nanopolymers (i.e., dendrimers) that

are multi-functionalized with reactive groups (e.g., Ti(IV) ions) for the highly 

specific recognition of phosphate groups, and with ‘reporting’ groups (e.g.,

biotin, fluorophore) that facilitate their detection.

Multiple functionalized groups per dendrimer ensure very strong binding to the

phosphoproteins (resulting in high specificity) and significant signal amplification

from many “reporting” groups (resulting in high sensitivity).

 

 

 

pIMAGO - Detection on Microplate 분석과정

 

 

pIMAGO-biotin Phosphoprotein Detection on Microplate Kits

All kits include pIMAGO-biotin reagent, streptavidin-based detection reagent, blocking buffer, pIMAGO buffer, high-binding adsorption 

plates, and a control phosphoprotein. HRP-based kits also include colorimetric substrate and stop solution

900-100      pIMAGO HRP Colorimetric PhosphoproteinDetection on Microplate (complete kit)       100 wells

900-400      pIMAGO HRP Colorimetric PhosphoproteinDetection on Microplate (complete kit)        400 wells

901-100      pIMAGO Fluor 550 Phosphoprotein Detectionon Western Blot (complete kit)       100 wells

901-400      pIMAGO Fluor 550 Phosphoprotein Detectionon Western Blot (complete kit)       400 wells

902-100      pIMAGO Fluor 680 Phosphoprotein Detectionon Western Blot (complete kit)       100 wells

902-400      pIMAGO Fluor 680 Phosphoprotein Detectionon Western Blot (complete kit)       400 wells

 

903-100      pIMAGO Fluor 800 Phosphoprotein Detectionon Western Blot (complete kit)       100 wells

903-400      pIMAGO Fluor 800 Phosphoprotein Detectionon Western Blot (complete kit)       400 wells

Protocols

•  pIMAGO-biotin for on-plate HRP detection – Protocol

•  pIMAGO-biotin for on-plate Fluor detection – Protocol

Technical Documents 

•  pIMAGO biotin HRP detection on microplate – 100 wells – Technical Document (Cat# 900-100)

•  pIMAGO biotin HRP detection on microplate – 400 wells – Technical Document (Cat# 900-400) 

•  pIMAGO biotin Fluor 550 detection on microplate – 100 wells – Technical Document (Cat# 901-100) 

•  pIMAGO biotin Fluor 550 detection on microplate – 400 wells – Technical Document (Cat# 901-400)

•  pIMAGO biotin Fluor 680 detection on microplate – 100 wells – Technical Document (Cat# 902-100)

•  pIMAGO biotin Fluor 680 detection on microplate – 400 wells – Technical Document (Cat# 902-400) 

•  pIMAGO biotin Fluor 800 detection on microplate – 100 wells – Technical Document (Cat# 903-100)

•  pIMAGO biotin Fluor 800 detection on microplate – 400 wells – Technical Document (Cat# 903-400)

 

MSDS

•  pIMAGO Kit MSDS