Deuterated Crosslinkers

Deuterated crosslinkers manufactured by ProteoChem are high quality deuterium-labeled crosslinkers, or "heavy" crosslinker analogs, for isotopically labeling protein and peptides in mass spectrometry (MS) research applications. ProteoChem's unique offering of ultrapure, deuterated crosslinking reagents allows researchers to rapidly analyze protein structure and protein-protein interactions.

Otherwise identical, heavy (d4 or d8) and light (d0) crosslinker analogs are simultaneously utilized in a 1:1 ratio during the crosslinking reaction. The crosslinked protein or protein complex can then be digested in preparation for mass spec analysis. The resulting crosslinked peptides with an isotopic label are readily identified in the mass spectrum as peaks that only differ by four or eight mass units.

 

 

Deuterated Crosslinker Selection Guide

 

 

 

1.  BS2G-d4

BS2G-d4 deuterated crosslinker, Bis(Sulfosuccinimidyl) 2,2,4,4-glutarate-d4, is a water soluble and cell membrane impermeable, protein crosslinker containing 4 deuterium atoms to provide a 4 dalton shift by mass spectrometry analysis. Use heavy BS2G-d4 deuterated crosslinking reagent in combination with the non-deuterated analog BS2G-d0 (BS2G-d0, BS2G) for mass spec studies. Together BS2G-d0 and BS2G-d4 can be used for protein-protein interaction mapping using Liquid Chromatography–Mass Spectrometry (LCMS, LC/MS, LC-MS).

 

•  Molecular Weight: 534.37

•  Spacer Length: 7.7Å

•  Alternative Names: Glutaric acid-2,2,4,4-d4 bis (3-sulfo-N-hydroxysuccinimide ester) disodium salt; D4-BS2G; BS2G-d4; d 4 -BS2G 

 

 

2.  BS3-d4

 

BS3-d4 deuterated crosslinking reagent, Bis(Sulfosuccinimidyl) 2,2,7,7-suberate-d4, is a water soluble and membrane impermeable, protein crosslinking agent with four deuterium atoms to provide a four dalton shift by mass spectrometry. Mass shifts are readily observed when heavy BS3-d4 is used in combination with the non-deuterated light BS3 crosslinking analog (BS3-d0) for mass spec studies.

 

 

•  Molecular Weight: 576.45

•  Spacer Length: 11.4Å

•  Alternative Name: Suberic acid-2,2,7,7-d4 bis (3-sulfo-N-hydroxysuccinimide ester) disodium salt; D4-BS3; BS3-d4; d 4 -BS3 

 

 

 

3.  DSG-d4

 

DSG-d4 deuterated crosslinking reagent (Bis[Succinimidyl] 2,2,4,4-glutarate-d4) is a cell membrane permeable, protein crosslinking agent with 4 deuterium atoms to provide a 4 dalton shift by mass spectrometry analysis. Use the heavy DSG-d4 deuterated protein crosslinking reagent in combination with the light, non-deuterated analog (DSG-d0) for mass spec studies.

 

•  Molecular Weight: 330.28

•  Spacer Length: 7.7 Å

•  Alternative Names: Glutaric acid-2,2,4,4-d4 bis (N-hydroxysuccinimide ester); DSG-d4 

 

 

 

4.  DSP-d8

 

DSP-d8 deuterated crosslinking reagent, Dithiobis(succinimidyl 2,2,3,3,6,6,7,7-propionate-d8), is a cell membrane permeable, homobifunctional, protein crosslinker containing eight deuterium atoms to provide an eight Dalton shift by mass spec compared to the non-dueterated DSP (DSP-d0). DSP-d8 deuterated crosslinking thakes place via amine-reactive NHS esters at both ends of a cleavable, 8-atom (12.0 angstrom) spacer arm. The deuterated crosslinking spacer arm contains a reducible disulfide bond (SS-bond) which makes it a good choice for numerous applications where reversible crosslinking is desired.

Use the heavy DSP-d8 deuterated protein crosslinker in combination with the light, non-deuterated analog (DSP-d0) for mass spec studies.

DSP-d8 deuterated crosslinker must be dissolved in an organic solvent, such as DMSO or DMF, and then may be added to an aqueous crosslinking reaction. Since DSP-d8 crosslinking reagent is cell membrane permeable it may be used to crosslink intracellular proteins.

 

•  Molecular Weight: 412.46

•  Spacer Length: 12.0 Å

•  Alternative names: DSP-d8; D8-DSP; Dithiobis(succinimidyl propionate-d8); DTSP-d8 

 

 

 

5.  DSS-d4

 

DSS-d4 deuterated crosslinker (Bis[Succinimidyl] 2,2,7,7-suberate-d4) is a cell membrane permeable crosslinking agent with four deuterium atoms that provide a four dalton shift by mass spectrometry analysis. Use DSS-d4 heavy crosslinker in combination with DSS crosslinker; the light, non-deuterated analog (DSS-d0) for mass spec studies.

•  Molecular Weight: 372.36

•  Spacer Length: 11.4 Å

•  Alternative Names: Suberic acid-2,2,7,7-d4 bis (N-hydroxysuccinimide ester); DSS-d4 

Cat. No	Product	Quantity
h5101-10mg	BS2G-d4	10 mg
h5101-50mg	BS2G-d4	50 mg
h5100-10mg	BS3-d4	10 mg
h5100-50mg	BS3-d4	50 mg
h5103-10mg	DSG-d4	10 mg
h5103-50mg	DSG-d4	50 mg
h5104-10mg	DSP-d8	10 mg
h5104-50mg	DSP-d8	50 mg
h5102-10mg	DSS-d4	10 mg
h5102-50mg	DSS-d4	50 mg