Protein Modification Reagents

Protein modification reagents are useful for adding labels to or blocking functional groups on proteins, peptides, and other molecules. SATA, HPG, Traut's Reagent and Sulfo-NHS-Acetate are some of the best and most effective protein modification reagents available.

 

1.  SATA

SATA protein modifier (N-Succinimidyl S-Acetylthioacetate) is a protein modification reagent used to convert primary amine groups into protected sulfhydryls (thiol; -SH) for later reaction with sulfhydryl reactive crosslinkers such as Sulfo-SMCC, Sulfo-MBS, etc. The sulfhydryl is readily made available for use in subsequent reactions after exposure to Hydroxylamine. After the simple deprotection step is performed to create the thioacetylated peptide, the free sulfhydryl containing peptide can then be conjugated to make a hapten-carrier conjugate, polymer, etc.

•  Molecular Weight: 231.23

•  Spacer Length: 2.8 ÅCAS Number: 76931-93-6

•  Alternative Names: N-Succinimidyl-S-Acetyl-Thioacetate; S-Acetylthioglycolic acid N-hydroxysuccinimide ester; N-Succinimidyl (acetylthio)acetate; N-Succinimidyl S-acetylthioglycolate; S-Acetylthioglycolic acid NHS ester

 

☞   SATA Protein Modification Protocol & Product Information Sheet

 

 

2.  Sulfo-NHS-Acetate

Sulfo-NHS-Acetate acylating agent (Sulfosuccinimidyl Acetate) is water soluble. Sulfo-NHS-Acetate irreversibly reacts with and blocks primary amines, such as in lysine, at pH greater than 7 to yield the acylated form. Using Sulfo-NHS-Acetate prevents polymerization when performing protein crosslinking reactions. Other Sulfo-NHS-Acetate uses include polymerization prevention when conjugating hapten peptides to carrier proteins for immunogen production.

•  Molecular Weight: 259.17

•  Spacer Length: 2.8 Å

•  CAS Number: 152305-87-8

•  Alternative Names: 1-acetyloxy-2,5-dioxopyrrolidine-3-sulfonic acid; 3-Pyrrolidinesulfonic acid, 1-hydroxy-2,5-dioxo-; 3-Sulfosuccinimid-1-yl acetate; 3-Sulfosuccinimid-1-yl acetate; 1-acetyloxy-2,5-dioxopyrrolidine-3-sulfonic acid 

 

☞   Sulfo-NHS-Acetate Protocol & Product Information Sheet

 

 

3.  Traut's Reagent (2-Iminothiolane)

Traut's Reagent (2-Iminothiolane HCl) is commonly used to convert primary amines into sulfhydryl groups in a one-step process is called thiolation. Traut's Reagent is water soluble. This protein modification reagent is amino reactive at pH 7-10 and is also an effective thiolation reagent for polysaccharides. In addition, Traut's Reagent is a useful RNA-protein crosslinking reagent.

 

•  Molecular Weight: 137.63

•  Spacer Length: 8.1 Å

•  CAS Number: 4781-83-3

•  Alternative Names: 2-Iminothiolane HCl; 2-Iminothiolane hydrochloride; 2-Iminothiolane; 2-IT; Trauts reagent 

 

 

4.  HPG

HPG (p-Hydroxyphenylglyoxal) is a protein modification reagent that reacts with arginine residues to give a large increase in absorbance at approximately 340 nm. HPG is a better choice for amino acid side chain modification due to its increased water solubility over phenylglyoxal and improved oxidation resistance over p-nitrophenylglyoxal.

 

•  Molecular Weight: 168.15

•  Chemical Formula: C8H6O3

•  CAS Number: 24645-80-5

•  Alternative Names: p-Hydroxyphenylglyoxal monohydrate; 4-hydroxyphenylglyoxal; (P-Hydroxyphenyl)Glyoxal; Benzeneacetaldehyde, 4-hydroxy-alpha-oxo-

 

☞   HPG Product Information Sheet & General Protocol

 

 

5.  Iodoacetic Acid

Iodoacetic Acid is ≥ 99.0% pure. Iodoacetic acid is used for the modification and alkylation of sulfhydryls (-SH) in cysteine residues found in proteins and peptides.

 

•  Molecular Weight: 185.95

•  CAS Number: 64-69-7

•  Linear Formula: ICH2CO2H

•  Alternative Names: Iodoacetate; 2-Iodoacetic Acid; IA; Iodoethanoic Acid; Monoiodoacetate; Monoiodoacetic acid; MIA 

 

 

6.  BMPA  

BMPA Modifier (N-ß-Maleimidopropionic acid) can be used as a protein crosslinker or sulfhydryl (-SH) to carboxylic acid (-COOH) modification reagent. As a protein modifier, BMPA is used to modify sulfhydryl groups at pH 6.5-7.5 to terminal carboxyl groups. BMPA protein modification makes -COOH groups for subsequent coupling via EDC crosslinking. As a protein crosslinker, BMPA can be used for the preparation of protein conjugates. The maleimide and carboxyl reactive groups of BMPA crosslinker are reactive toward sulfhydryls and hydrazides/amines, respectively.

 

•  Spacer Arm Length: 5.9 Å

•  Molecular Weight: 169.13

•  CAS Number: 7423-55-4

•  Chemical Formula: C7H7NO4

•  Alternative Names: beta-Maleimidopropionic Acid; b-Maleimidopropionic Acid; 3-Maleimidopropionic Acid 

Cat.No	Product	Quantity
m3100-100mg	SATA Protein Modifier	100 mg
m3100-1gm	SATA Protein Modifier	1 gram
m3102-100mg	Sulfo-NHS-Acetate	100 mg
m3102-1gm	Sulfo-NHS-Acetate	1 gram
m3103-500mg	Traut's Reagent (2-Iminothiolane)	500 mg
m3103-500mg	Traut's Reagent (2-Iminothiolane)	1 gram
m3101-100mg	HPG	100 mg
m3101-1gm	HPG	1 gram
m3104-1gm	Iodoacetic Acid	1 gram
m3105-100mg	BMPA Modifier	100 mg
m3105-100mg	BMPA Modifier	100 mg